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文献和实验Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM
Materials: see Solutions for Recipes TE buffer 10% (w/v) sodium dodecyl sulfate (SDS ) 20 mg/ml proteinase K
immediately to Eluting DNA. 4. Eluting DNA 1) Set a water bath to 65°C. 2) Remove the plate containing the pelleted magnetic beads (Step 11, above) from the Magnetic Separator. 3) Add 250 µl Elution Buffer
Automated Genomic DNA Extraction
genomic DNA from 10-20 µl blood samples. The volumes given are on a per sample basis. Start with 96 x 10-20 µl blood samples in a 96 x 2 ml deep well plate. Add 500 µl of Lysis Mix and incubate at room temperature for 10 minutes
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