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- 英文名：
Direct Genomic DNA Sequencing, w/o prep
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文献和实验Grow 10ml YPD cultures o/n. Figure out cell density; inoculate 30 ml YPD and grow o/n so that cell density is approximately 2 x 108 cells/ml the next morning. Spin down cells in 50 ml sterile conical tubes for 10 min. Centrifugation
Advances in DNA sequencing techniques have made it possible to routinely sequence long DNA molecules (1 ,2 ). Recently double-stranded DNA has been directly sequenced with chain terminators (3 ), and this eliminates the tedious task
Genomic DNA prep (Jorgensen Lab [modified slightly by Mike H.]) Make worm growth plates. These use agarose to avoid impurities in most batches of agar, and are enriched to allow greater worm growth. Mix: 5 g Bacto
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