- 询价
- Thermo scientific -fermentas
- K0721
- 2025年10月11日
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| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| K0721 | for 50 preps. | K0721 | |
| K0722 | for 250 preps. | K0722 |
纯化操作流程
- Features
-
- Universal – can be used for both cell and tissue samples from a wide range of sources.
- Efficient – high yields of high molecular weight genomic DNA.
- Fast – 20 minute procedure following cell lysis.
- Convenient – spin columns are capped and assembled with collection tubes.
- Applications
-
- Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
– PCR and qPCR,
– FastDigest® and conventional restriction digestion,
– Southern blotting.
- Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.
The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA of more than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting and enzymatic reactions.
- Proteinase K Solution
- RNase A Solution
- Digestion Solution
- Lysis Solution
- Wash Buffer I (concentrated)
- Wash Buffer II (concentrated)
- Elution Buffer
- GeneJET™ Purification Columns (pre-assembled with collection tubes)
- Collection tubes
- Detailed Protocol
150 ng of isolated genomic DNA from each source was fractionated by agarose gel (0.5%) electrophoresis.
M – Lambda DNA/HindIII Marker (#SM0101)
1 – human bone marrow
2 – human blood
3 – rat spleen
4 – mouse heart
5 – mouse liver
6 – HeLa cells
7 – Jurkat cells
8 – E.coli DH5alfa cells
| Source | Quantity | Yield, µg |
| Mammalian blood | 200 µl | 4-6 |
| Mouse heart | 10 mg | 10-15 |
| Mouse tail | 0.5 cm | 8-10 |
| Rat liver | 10 mg | 10-20 |
| Rat spleen | 5 mg | 20-30 |
| Rat kidney | 10 mg | 25-30 |
| Rabbit ear | 20 mg | 5-10 |
| Bacillus pumilis cells | 2x109 cells | 10-15 |
| Escherichia coli cells | 2x109 cells | 10-15 |
| HeLa cells | 2x106 cells | 15-20 |
| Jurkat cells | 5x106 cells | 25-30 |
| Saccharomyces cerevisiae cells | 1x108 cells | 3-5 |
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文献和实验Reproducible purification of DNA and RNA in 96-well format. Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. A Purified DNA samples were analyzed on a 0.8% agarose gel. B
Purification of Genomic DNA Using PureLink Silica Columns
different tubes. Before Starting Add 40 ml 96-100% ethanol to 10 ml Wash Buffer (W5) included with the kit. Store the Wash Buffer (W5) with ethanol at room temperature. Binding DNA 1. Remove a PureLink™ Spin Cartridge
) for 1 minute to elute DNA. 12) For genomic DNA clean up, repeat elution step 10-11 by adding another 15-20 ul Elution Buffer. 2. MicroElute™ DNA Clean-Up Vacuum/Spin Protocol Note: Please read through previous section before using
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