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The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.
The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA of more than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting and enzymatic reactions.
Source | Quantity | Yield, µg |
Mammalian blood | 200 µl | 4-6 |
Mouse heart | 10 mg | 10-15 |
Mouse tail | 0.5 cm | 8-10 |
Rat liver | 10 mg | 10-20 |
Rat spleen | 5 mg | 20-30 |
Rat kidney | 10 mg | 25-30 |
Rabbit ear | 20 mg | 5-10 |
Bacillus pumilis cells | 2x109 cells | 10-15 |
Escherichia coli cells | 2x109 cells | 10-15 |
HeLa cells | 2x106 cells | 15-20 |
Jurkat cells | 5x106 cells | 25-30 |
Saccharomyces cerevisiae cells | 1x108 cells | 3-5 |
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