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- Thermo scientific -fermentas
- K0721
- 2025年10月11日
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| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| K0721 | for 50 preps. | K0721 | |
| K0722 | for 250 preps. | K0722 |
纯化操作流程
- Features
-
- Universal – can be used for both cell and tissue samples from a wide range of sources.
- Efficient – high yields of high molecular weight genomic DNA.
- Fast – 20 minute procedure following cell lysis.
- Convenient – spin columns are capped and assembled with collection tubes.
- Applications
-
- Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
– PCR and qPCR,
– FastDigest® and conventional restriction digestion,
– Southern blotting.
- Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
说明
The GeneJET™ Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast (see Fig.1, Table 1).
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.
The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA of more than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting and enzymatic reactions.
质量控制
The GeneJET™ Genomic DNA Purification Kit is qualified by isolating genomic DNA from 200 µl of blood and 5 mg of mammalian tissue according to the recommended protocols. The purified genomic DNA has an A260/280 ratio of ≥1.7. A single band of more than 30 kb is seen after agarose gel electrophoresis and ethidium bromide staining. Purified genomic DNA is evaluated by PCR amplification of a single-copy gene and digestion with FastDigest® restriction enzymes.
组分
- Proteinase K Solution
- RNase A Solution
- Digestion Solution
- Lysis Solution
- Wash Buffer I (concentrated)
- Wash Buffer II (concentrated)
- Elution Buffer
- GeneJET™ Purification Columns (pre-assembled with collection tubes)
- Collection tubes
- Detailed Protocol
图1。Genomic DNA from various sources purified using GeneJET™ Genomic DNA Purification Kit.
150 ng of isolated genomic DNA from each source was fractionated by agarose gel (0.5%) electrophoresis.
M – Lambda DNA/HindIII Marker (#SM0101)
1 – human bone marrow
2 – human blood
3 – rat spleen
4 – mouse heart
5 – mouse liver
6 – HeLa cells
7 – Jurkat cells
8 – E.coli DH5alfa cells
150 ng of isolated genomic DNA from each source was fractionated by agarose gel (0.5%) electrophoresis.
M – Lambda DNA/HindIII Marker (#SM0101)
1 – human bone marrow
2 – human blood
3 – rat spleen
4 – mouse heart
5 – mouse liver
6 – HeLa cells
7 – Jurkat cells
8 – E.coli DH5alfa cells
图2。Yields of genomic DNA purified using GeneJET™ Genomic DNA Purification Kit and kits of other vendors.
表1。Typical genomic DNA yields from various sources.
| Source | Quantity | Yield, µg |
| Mammalian blood | 200 µl | 4-6 |
| Mouse heart | 10 mg | 10-15 |
| Mouse tail | 0.5 cm | 8-10 |
| Rat liver | 10 mg | 10-20 |
| Rat spleen | 5 mg | 20-30 |
| Rat kidney | 10 mg | 25-30 |
| Rabbit ear | 20 mg | 5-10 |
| Bacillus pumilis cells | 2x109 cells | 10-15 |
| Escherichia coli cells | 2x109 cells | 10-15 |
| HeLa cells | 2x106 cells | 15-20 |
| Jurkat cells | 5x106 cells | 25-30 |
| Saccharomyces cerevisiae cells | 1x108 cells | 3-5 |
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GeneJET™ Genomic DNA Purification Kit
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