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Phospho-Histone H3 (Ser10) (D2

C8) XP™ Rabbit mAb
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  • 询价
  • Cell Signaling Technology已认证
  • 3377
  • USA
  • 2025年07月16日
  • western blot,免疫荧光（IF）,流式细胞（Flow Cyt）
  • 兔
  • 人,小鼠,大鼠,驴,斑马鱼
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商：

      CST

    • 抗原：

      /

    • 保存条件：

      -20°c

    • 库存：

      大量

    • 宿主：

      兔

    • 级别：

      详见MSDS文件

    • 抗体英文名：

      Phospho-Histone H3 (Ser10) (D2C8) XP Rabbit mAb

    • 是否单克隆：

      1

    • 适应物种：

      人,小鼠,大鼠,驴,斑马鱼

    • 应用范围：

      western blot,免疫荧光（IF）,流式细胞（Flow Cyt）

    • 抗原来源：

      /

    • 保质期：

      详见说明书

    • 规格：

      40 ul (4 western blots)/100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free &amp; custom formulation / quantity</a> /40 ul (4 western blots)/100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free &amp; custom formulation / quantity</a>

    规格：产品价格：￥询价
    规格：40 ul (4 western blots)产品价格：￥请询价
    规格：100 ul (10 western blots)产品价格：￥请询价
    规格：<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free &amp; custom formulation / quantity</a> 产品价格：￥请询价
    规格：40 ul (4 western blots)产品价格：￥请询价
    规格：100 ul (10 western blots)产品价格：￥请询价
    规格：<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free &amp; custom formulation / quantity</a> 产品价格：￥请询价
    XP Monoclonal Antibody

    Product Pathways - Chromatin Regulation / Epigenetics

    Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377

    Applications Reactivity Sensitivity MW (kDa) Isotype
    W IF-IC F H M R Mk Z Endogenous 17 Rabbit IgG

    Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
    Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Z=Zebrafish
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Protocols

    Specificity / Sensitivity

    Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb detects endogenous levels of histone H3 only when phosphorylated at Ser10. The antibody does not cross-react with other phosphorylated histones or with acetylated histones.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser10 of human histone H3.

    Western Blotting

    Western Blotting

    Western blot analysis of extracts from HeLa cells, either untreated or treated with nocodazole (0.1 mg/ml for 18 hours), using Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377 (upper) or Histone H3 Antibody #9715 (lower). Phospho-specificity of the antibody is shown by further treatment of the lysate with λ phosphatase.

    Flow Cytometry

    Flow Cytometry

    Flow cytometric analysis of Jurkat cells using Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb versus propidium iodide (DNA content). The boxed population indicates Phospho-Histone H3 (Ser10) positive cells.

    IF-IC

    IF-IC

    Confocal immunofluorescent analysis of HeLa cells using Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


    Background

    Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

    1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
    2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
    3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
    4. Cheung, P. et al. (2000) Cell 103, 263-71.
    5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
    6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
    7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
    8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
    9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
    10. Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
    11. Dai, J. et al. (2005) Genes Dev 19, 472-88.

    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    For Research Use Only. Not For Use In Diagnostic Procedures.

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      ：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫

    • RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

      /well, 6 well plate) or 4×106/10cm plate were transfected with 5' biotin linked EF52 sense, antisense or sense/antisense RNAs (100nM). Twenty-four hours later the cultures were isolated, washed 1 time in 1x PBS, genomic DNA isolated (Qiagen™ Dneasy), and exposed

    • 做好 ChIP 的四个注意事项

      2B12) XP® Rabbit mAb (ChIP Formulated) #4620。该抗体可检测与基因组中所有 DNA 序列结合的所有组蛋白 H3 类型（H3、H3.3、CENP-A）。因此，不管检测的位点活性状态如何，该抗体都能为 ChIP 实验提供通用的阳性对照。阴性对照阴性对照抗体（如正常兔 IgG）不能识别特异抗原表位，因此可用于检测非特异性结合。例如，如果阴性对照样品中的产物量等于特异靶标样品中产物量，那么可得出结论，特异靶标抗体出现非特异结合或本底水平的信号。这个结果结合阳性组蛋白 H3

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