- 询价
- Cell Signaling Technology
- 5251
- USA
- 2025年10月06日
- W, IHC-P, IF-IC, F
- Rabbit
- H,M,R,Mk
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- 详细信息
- 技术资料
- 抗体英文名：
Phospho-TCTP (Ser46) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser46 of human TCTP protein
- 应用范围：
W, IHC-P, IF-IC, F
- 宿主：
Rabbit
- 供应商：
CST
- 级别：
详见MSDS文件
- 适应物种：
H,M,R,Mk
- 保质期：
详见说明书
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P IF-IC F | H M R Mk | Endogenous | 23 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-TCTP (Ser46) Antibody recognizes endogenous levels TCTP protein only when phosphorylated at Ser46. In some cell types the antibody may recognize an 80-100 kDa protein of unknown origin. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser46 of human TCTP protein. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from U-2 OS cells, untreated or synchronized in mitosis by thymidine block followed by release into nocodazole, using Phospho-TCTP (Ser46) Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or lambda-phosphatase-treated (right), using Phospho-TCTP (Ser46) Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HT-29 cell pellets, control (left) or nocodazole-treated (right), using Phospho-TCTP (Ser46) Antibody. Flow Cytometry
Flow cytometric analysis of Jurkat cells using Phospho-TCTP (Ser46) Antibody compared to propidium iodide (DNA content). IF-IC
Confocal immunofluorescent analysis of U-2 OS cells using Phospho-TCTP1 (Ser46) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | Translationally controlled tumor protein (TCTP/p23/HRF) is a ubiquitously expressed and highly conserved protein involved in various cellular processes, such as its role as a histamine releasing factor in chronic allergic disease (1). TCTP binds tubulin in a cell cycle dependent manner and is associated with the mitotic spindle (2). In addition, TCTP interacts with the actin cytoskeleton to regulate cell shape (3). In mitosis, TCTP is phosphorylated by PLK at Ser46, decreasing microtubule stability (4,5). TCTP interacts with the small GTPase Rheb, possibly acting as a GEF, thereby activating the TORC1 pathway and controlling cell growth and proliferation (6,7). TCTP has also been shown to be involved in apoptosis and cell stress (8-11). In cultured cells, reduction in TCTP expression can cause loss of the malignant phenotype (12).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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