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- 英文名:
EZ DNA Methylation Kit
- 供应商:
简石生物
HIGHLIGHTS
- Streamlined, proven procedure for bisulfite conversion of DNA.
- Desulphonation and recovery of bisulfite-treated DNA with a spin column.
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
DESCRIPTION
The EZ DNA Methylation Kit features a simplified procedure that streamlines bisulfite treatment of DNA. This kit is the original bisulfite conversion kit from Zymo Research. The EZ DNA Methylation Kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite during which cytosine is converted into uracil. Innovative desulphonation technologies eliminate otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including library preparation for Next-generation sequencing, PCR amplification, endonuclease digestion, sequencing, microarrays, etc. The EZ DNA Methylation Kit is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
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文献和实验• 以96孔板方式,从同一个细胞或组织样本中同时纯化基因组 DNA和总RNA • 处理 96 个样本,只需不到60 分钟 • 从同一样本中纯化高品质的基因组DNA和总RNA • 96孔板方式,高效的操作流程 • 高度标准化的操作流程 • 高重复性的DNA和RNA产量 Product description The AllPrep DNA/RNA 96 Kit is well suited for high
大伙好,我购买Chemicon公司的CpGenome DNA Modificatio Kit 打算五月开始做,希望以后一起交流一下甲基化分析的实验技术。 QQ:395848659 Here are some online resources for methylation study CpG island prediction: CpG Island Searcher CpG Plot MethPrimer CpGProD (CpG Island
,这种甲基化方式具可遗传性。整个基因约有30,000个CPG岛,其中50~60%与基因有关,分布于促进子(promoter)内。研究证明:促进区内CPG岛的甲基化与基因的转录抑制有关。DNA甲基化对发育是非常重要的。三种DNA甲基化转移酶(DNMT1、DNMT3A、DNMT3B)的任一纯合性丢失可导致小鼠的死亡,提示DNA甲基化对基因的正确表达是非常重要。但成年组织对甲基化的依赖程度较低,其作用可能是维持大量非编码区处于失活状态,以保证转录因子(TF)与其靶位点特异结合。CPG岛甲基化作为一种控制











