- 询价
- ZYMO RESEARCH
- 美国
- D5013
- 2025年12月27日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20C
- 英文名：
Human Methylated & Non-Methylated (WGA) DNA Set
- 供应商：
简石生物
HIGHLIGHTS
- Purified, non-methylated and methylated human DNA for use as negative and positive control in methylation detection applications.
- Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency.
- Ideal controls for bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP). Primers included with the full set are designed to amplify non-methylated, methylated, and mixed methylated copies of the Ras association (RalGDS/AF-6) domain family member 1 (RASSF1) gene following bisulfite conversion.
DESCRIPTION
The Human WGA Methylated & Non-methylated DNA Set consists of two control DNAs (non-methylated and methylated) along with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation, EZ DNA Methylation-Gold, EZ DNA Methylation-Direct, and EZ DNA Methylation-Lightning kits from Zymo Research to assess the efficiency of bisulfite-mediated conversion of DNA. The Human WGA Methylated & Non-methylated DNA Set is generated using phi29 DNA polymerase based whole genome amplification techniques from HCT116 DKO cell line derived genomic DNA (Human HCT116 DKO Non-methylated DNA). The Human WGA Methylated DNA is Human WGA Non-methylated DNA that has been enzymatically methylated at all double-stranded CG dinucleotides using M.SssI methyltransferase and can be used as a positive control for DNA methylation analysis.
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文献和实验Methylated DNA Immunoprecipitation (MeDIP)
Introduction Methylation of cytosines can mediate epigenetic gene silencing and is the most prominent DNA modification in eukaryotes. MeDIP is an immunocapturing approach to enrich DNA that is methylated. The principle
Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation
to methylated DNA. Methyl-CpG Immunoprecipitation (MCIp) employs an immobilized recombinant human methyl-CpG binding domain protein 2, MBD2, which binds methylated CpGs in double-stranded DNA. Elution with increasing salt concentrations allows the fractionated
Whole Genome Methylation Profiling by Immunoprecipitation of Methylated DNA
I provide a protocol for DNA methylation profiling based on immunoprecipitation of methylated DNA using commercially available monoclonal antibodies that specifically recognize 5-methylcytosine. Quantification of the level of enrichment
