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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
常温
- 英文名:
ssDNA/RNA Clean & Concentrator
- 供应商:
简石生物
HIGHLIGHTS
- Quick and Reliable: 10 minute clean-up and concentration of ssDNA/RNA (17-200 nt).
- Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
- Clean and Pure: ssDNA/RNA ready for downstream applications like PCR, RT-qPCR, etc.
DESCRIPTION
The ssDNA/RNA Clean & Concentrator provides a simple and reliable method for the rapid separation, clean-up, and concentration of 10 µg per prep of single stranded DNA and/or RNA (e.g., short transcripts, probes, primers) from double stranded species (e.g., genomic DNA).This simple 10 minute procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology. Single stranded DNA or RNA ≥ 17 to 200 nucleotides (e.g., short transcripts, probes, primers) can be safely treated and recovered using this kit. The result is highly concentrated, purified ssDNA/RNA that is suitable for subsequent molecular methods including PCR, RT-PCR, hybridization, etc.
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文献和实验RNA Purification Protocol for 1-2 x 107 cultured cells
temperature. 8. Pour of the supernatant and drain the tube briefly on a clean absorbent paper. Add 3 ml of 70% ethanol and invert the tube few times to wash the RNA pellet. 9. Centrifuge at 3,000-5,000 x g for 10 minutes at room temperature
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
μL) into new 1.5 ml centrifuge tube for RNA/DNA isolation. Remove any liquid from the original tube contains protein precipitate by invert the plate on a absorbent paper. Keep the tube contains protein pellet for protein isolation start from step 26
Differentiation of embryonic stem cells is accompanied by changes of gene expression and chromatin and chromosome dynamics. One of the most impressive examples for these changes is inactivation of one of the two X chromosomes occurring












