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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse PD-L1 / B7-H1 / CD274 Protein (ECD, Fc Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/100.00 µg/200.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥15050.0 |
|---|---|---|---|
| 规格: | 100.00 µg | 产品价格: | ¥2310.0 |
| 规格: | 200.00 µg | 产品价格: | ¥3870.0 |
蛋白名称:PD-L1蛋白, PD-L1 protein
蛋白构建:A DNA sequence encoding the mouse Cd274 (NP_068693.1) (Met1-His239) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:1.Measured by its binding ability in a functional ELISA. 2.Immobilized human PD1-His(Cat:10377-H08H) at 10μg/mL (100μL/well) can bind mouse CD274-Fc(Cat:50010-M02H), the EC50 of mouse CD274-Fc is 0.1-0.6μg/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Phe 19
蛋白分子量:The recombinant mouse Cd274 consists 459 amino acids and predicts a molecular mass of 51.6 kDa.
蛋白NP号:NP_068693.1
蛋白氨基酸序列:Met1-His239
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验,PD-1/PD-L1这么值钱,大家一定好奇它到底是什么鬼。 PD-1全名是程序性死亡分子1(programmed death-1)是Ishida等[1]通过消减杂交技术于1992年发现的[2],是CD28家族成员,胞质区含有2个酪氨酸残基,1个位于靠近N端的免疫受体酪氨酸抑制基序(ITIM)中,另一个1个位于靠近C端的免疫受体酪氨酸转化基序(ITSM)中[2]。 PD-L1(CD274)是B7家族成员,是PD-1的配体,属于1型跨膜蛋白,全长290个氨基酸,包含1个IgV样
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
Arabidopsis protein microarrays. Proc. Natl. Acad. Sci. U.S.A. 104:4730‐4735. Ptacek, J., Devgan, G., Michaud, G., Zhu, H., Zhu, X., Fasolo, J., Guo, H., Jona, G., Breitkreutz
in complex organisms. Bioessays 25:930‐939. Mi, J., Zhang, X., Rabbani, Z.N., Liu, Y., Su, Z., Vujaskovic, Z., Kontos, C.D., Sullenger, B.A., and Clary, B.M. 2006. H1 RNA
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