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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse IL-1 beta / IL1B Protein
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/5.00 µg/20.00 µg/100.00 µg/500.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥29180.0 |
|---|---|---|---|
| 规格: | 5.00 µg | 产品价格: | ¥780.0 |
| 规格: | 20.00 µg | 产品价格: | ¥1980.0 |
| 规格: | 100.00 µg | 产品价格: | ¥4980.0 |
| 规格: | 500.00 µg | 产品价格: | ¥18980.0 |
蛋白名称:IL-1 beta蛋白, IL-1 beta protein
蛋白构建:A DNA sequence encoding the mature form of mouse IL1β (P10749) (Val 118-Ser 269) was expressed, with an initial Met at the C-terminus.
表达宿主:E. coli
蛋白纯度:> 97 % as determined by SDS-PAGE
蛋白活性:1. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 6-30 ng/mL.. 2. Measured by its ability to bind human IL1R1 in a functional ELISA. 3. Measured by its ability to bind mouse IL1R1 in a functional ELISA.
蛋白内毒素:Please contact us for more information.
预测N端:Met
蛋白分子量:The recombinant mouse IL1β consists of 153 amino acids and has a predicted molecular mass of 17.5 kDa. It migrates as an approximately 19 kDa band in SDS-PAGE under reducing conditions as predicted.
蛋白NP号:P10749
蛋白氨基酸序列:Val118-Ser269
蛋白标签:Native
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Du, Y; et al. Cancer cell-expressed BTNL2 facilitates tumour immune escape via engagement with IL-17A-producing γδ T cells. Nature communications, PMID: 35017553
3, Yun, X; et al. Madecassic acid alleviates colitis-associated colorectal cancer by blocking the recruitment of myeloid-derived suppressor cells via the inhibition of IL-17 expression in γδT17 cells. Biochemical pharmacology, PMID: 35700756
4, Won, T; et al. Cardiac myosin-specific autoimmune T cells contribute to immune-checkpoint-inhibitor-associated myocarditis. Cell reports, PMID: 36351411
5, Qiu, D; et al. Myeloid Cell-Derived IL-1 Signaling Damps Neuregulin-1 from Fibroblasts to Suppress Colitis-Induced Early Repair of the Intestinal Epithelium. International journal of molecular sciences, PMID: 38674054
6, Feng, X; et al. Synergistic therapeutic effect of parecoxib and ilomastat combination in osteoarthritis via inhibition of IL-17/PI3K/AKT/NF-κB activity. Molecular immunology, PMID: 39933417
7, Liu, B; et al. Targeted Blockage of Pathological Extracellular Vesicles and Particles From Fibroblast-Like Synoviocytes for Osteoarthritis Relief: Proteomic Analysis and Cellular Effect. Journal of extracellular vesicles, PMID: 40919882
下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。
正文炎症等免疫研究中 IL-1 家族的成员是重要的研究对象。这些促炎症和抗炎症因子在有机体应对病原感染和损伤时扮演了重要作用。IL-1β 有其自身鲜明的特点,实验前需要多了解。下面让我们来一一解读。1. 靶标生物学特点IL-1β 是 IL-1 家族众多成员中研究的最为深入的成员。包括免疫细胞(如单核细胞和巨噬细胞)在内的很多细胞都可以产生并分泌。IL-1β 的释放大致分为 3 个步骤:i)无活性前体的产生;ii)前体的成熟活化;iii)活化蛋白向胞外的分泌。众多刺激都会诱导这个过程,可分为病原
近年来,重组蛋白的应用有了显著增长,与此同时,用于重组蛋白表达和纯化的技术和产品也得以增长。自从亲和标签融合技术出现以来,多种多样的短肽或蛋白亲和标签极大地方便了外源表达重组蛋白的分离与蛋白质复合体的纯化,已成为蛋白质研究领域不可或缺的工具。多聚组氨酸标签(His-tag)是目前高通量蛋白纯化最普遍使用的亲和标签,His 标签融合蛋白的固定化金属离子亲和层析(immobilized metal-ion affinity chromatography, IMAC)被作为主要的蛋白纯化方法。从重组蛋白
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