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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse CD64 / FCGR1 Protein (His & AVI Tag), Biotinylated
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20.00 µg/50.00 µg/100.00 µg
| 规格: | 20.00 µg | 产品价格: | ¥2980.0 |
|---|---|---|---|
| 规格: | 50.00 µg | 产品价格: | ¥3870.0 |
| 规格: | 100.00 µg | 产品价格: | ¥6880.0 |
蛋白名称:CD64蛋白, CD64 protein
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Pro 297) of mouse FCGR1 (NP_034316.1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA. Immobilized human IgG1 at 10 μg/ml (100 μl/well) can bind biotinylated mouse CD64-AVI. The EC50 of biotinylated human IgG1 is 0.1-0.5 μg/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu 25
蛋白分子量:The recombinant mouse FCGR1 consists of 308 amino acids after removal of the signal peptide and has a predicted molecular mass of 34.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FCGR1 is approximately 50-55 kDa due to glycosylation.
蛋白NP号:NP_034316.1
蛋白氨基酸序列:Met1-Pro297
蛋白标签:C-AVI & His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验V294.PART II,Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
). 4. In addition to studying the response of BMMs isolated from knock-out mice, theDCC can be used to characterize the chemotactic response of cells expressingtagged proteins introduced either by microinjection of recombinant protein ordeoxyribonucleic acid (16
前髓细胞性白血病,是急性髓样白轿病的一种独特的亚型(FABM3),具有t[15;17][q22;q12-21]易位的特征,使染色体15上被称为PML的转录因子与维甲类细胞核受体α[RARα]融合,导致在APL细胞中表达PML/RARα融合性蛋白。 Adaptor protein 适配子(衔接子)蛋白,一种不含央在酶活性的蛋白质,但含有多种蛋白-蛋白相互作用域,如SH2/SH3域作为活化的细胞表面受体与各种细胞内信号转导蛋白之间的桥梁,把细胞膜活化复合物中的蛋白质连在一起。
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