- ￥1790 - 3220
- Sino Biological
- 北京
- 10204-H02H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human E-Cadherin / CDH1 / E-cad / CD324 Protein (Fc Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
50.00 µg/100.00 µg
| 规格： | 50.00 µg | 产品价格： | ￥1790.0 |
|---|---|---|---|
| 规格： | 100.00 µg | 产品价格： | ￥3220.0 |
蛋白名称：E-Cadherin蛋白, E-Cadherin protein
蛋白构建：A DNA sequence encoding the human E-Cad (P12830)(Met1-Ile707) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When cells are added to E-Cad coated plates (5 μg/mL, 100 μL/well), approximately 33% will adhere specifically after 90 minutes at 37 ℃.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 23 & Asp 155
蛋白分子量：The recombinant human E-Cad/Fc is a disulfide-linked homodimer. The reduced monomer comprises 791 amino acids and has a predicted molecular mass of 87.1 kDa. The apparent molecular mass of the protein is approximately 116-126 kDa in SDS-PAGE under reducing conditions.
蛋白NP号：P12830
蛋白氨基酸序列：Met1-Ile707
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Schimmel L, et al. Epithelial cell spreading assay on E-cadherin-coated glass or PDMS substrates for microscopy-based analysis of cadherin adhesions.STAR protocols, PubMed ID: 37792537
3, Kim SH, et al. Altering Cell Junctional Tension in Spheroids through E-Cadherin Engagement Modulation.ACS applied bio materials, PubMed ID: 38729097
Cell Surface Immunofluorescence Staining Protocol
. #420201). If using in vitro stimulated cells, simply resuspend previously activated cultures in Cell Staining Buffer and proceed to Step 2. 2) Add Cell Staining Buffer up to ~15 ml and centrifuge at 350 x g for 5 minutes, discard supernatant
.7. Wang, X. et al. 2011. Study of two G-protein coupled receptor variants of human trace amine-associated receptor 5. Sci Rep1: 102 (DOI:10.1038/srep00102).8. Wang, X. and Zhang, S. 2011. Production of a bioengineered G-protein coupled receptor of human
100-18B Human FGF-basic，10mM Tri(pH 8.0)用于溶解100-25 Human FGF-8。 关于PeproTech公司重组细胞因子和蛋白溶解的具体方法和注意事项请见：http://www.biomart.cn/experiment/430/488/490/492/31321.htm PeproTech公司简介： PeproTech Inc. 创立于1988年，始终致力于为生命科学研究开发重组蛋白及相关产品
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