Recombinant Human E-Cadherin / CDH1 / E-cad / CD324 Protein (ECD, His Tag) | 重组人 E-Cadherin / CDH1 / E-cad / CD324 蛋白 (ECD, His标签)
- ￥3220 - 21030
- Sino Biological
- 北京
- 10204-H08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human E-Cadherin / CDH1 / E-cad / CD324 Protein (ECD, His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100 µg/1 mg
| 规格： | 100 µg | 产品价格： | ￥3220.0 |
|---|---|---|---|
| 规格： | 1 mg | 产品价格： | ￥21030.0 |
蛋白名称：Human E-Cadherin / CDH1 / E-cad / CD324 Protein (ECD, His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain of human E-Cad precusor (NP_004351.1) (Met 1-Ile 707) was fused with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When cells are added to E-Cad coated plates (5 μg/mL, 100 μL/well), approximately 20%-40% of cells will adhere specifically after 90 minutes at 37 ℃.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 23 & Asp 155
蛋白分子量：The recombinant pro form of human E-Cad consists of 696 amino acids and has a calculated molecular mass of 77 kDa. As a result of glycosylation, the apparent molecular mass of rh E-Cad is approximately 90.6 kDa corresponding to the mature form and pro form respectively in SDS-PAGE under reducing conditions.
蛋白NP号：NP_004351.1
蛋白氨基酸序列：Met1-Ile707
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, CJ Pendegrass; et al. The effect of adherens junction components on keratinocyte adhesion
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High Throughput Production of Recombinant Human Proteins for Crystallography
, to be applied based on specific knowledge of protein families or when the core protocol is unsatisfactory. This scheme has been applied to more than 550 human proteins (>10,000 constructs) and has resulted in the deposition of 112 unique structures
Isolate or Deplete Human CD4+ T Cells - Whole Blood, Buffy Coat, MNC
/buffy coat to remove interfering soluble factors. （1）Dilute the whole blood or buffy coat in Buffer 1 (1 2). （2） Centrifuge at 600 x g for 10 min at 2-8°C. （3） Discard the plasma fraction/upper layer. Resuspend blood to the original
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
, H. 2009. Profiling the human protein‐DNA interactome reveals ERK2 as a transcriptional repressor of interferon signaling. Cell 139:610‐622. Hudson, J.R., Jr., Dawson, E.P
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