Recombinant Human APEX1 / AP / APEx / Ref-1 Protein (His Tag) | 重组人 APEX1 / AP / APEx / Ref-1 蛋白 (His标签)
- ￥4520
- Sino Biological
- 北京
- 12367-H07E
- 2025年08月12日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human APEX1 / AP / APEx / Ref-1 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100 µg
蛋白名称：APE1/APEX1蛋白, APE1/APEX1 protein
蛋白构建：A DNA sequence encoding the human APEX1 (P27695) (Pro2-Leu 318) was expressed, with a polyhistidine tag at the N-terminus.
表达宿主：E. coli
蛋白纯度：> 92 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met
蛋白分子量：The recombinant human APEX1 consisting of 328 amino acids and migrates as an approximately 37 kDa band in SDS-PAGE under reducing conditions as predicted.
蛋白NP号：P27695
蛋白氨基酸序列：Pro2-Leu318
蛋白标签：N-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
Co-Expression of human DNA primase p49-p58 subunits
resistance)and pACYC184-Hp58 (low copy,chloramphenicol resistance)from Fanning lab.Despite what the plasmid names might suggest,Hp49 is expressed native,while Hp58 is expressed w/an NT his tag (see Copeland ref). Antibiotic concentrations used: [AMP] = 100
Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
complexes from human HeLa S3 cells in a scale and purity optimized for characterization by mass spectrometry. For this purpose, we use stably expressed One-STrEP -tag ® fusion proteins. This approach was successfully used in characterization of histone
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