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- 保质期：
1年以上
- 英文名：
4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI)
- 库存：
大量
- 供应商：
solarbio
- CAS号：
28718-90-3
- 保存条件：
−20℃
- 规格：
10mg
产品名称：4',6-二脒基-2-苯基吲哚（DAPI）可用于细胞核染色的试剂
英文名称: 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI)
说明：DNA链荧光染色。
分子式：C16H15N5 ·2HCl
分子量：350.25
CAS＃：28718-90-3
外观：黄色结晶性粉末
特性：纯度：≥90% (HPLC和TLC)
溶解性：溶于水，参考浓度20mg/ml，加热有助于溶解。
储存条件：−20℃避光保存
DAPI是一种可以穿透细胞膜的蓝色荧光染料。和双链DNA结合后可以产生比DAPI自身强20多倍的荧光。和EB（ethidium bromide）相比，对双链DNA的染色灵敏度要高很多倍。DAPI染色常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常用于普通的细胞核染色以及某些特定情况下的双链DNA染色。
化学性质
DAPI是一种可以穿透细胞膜的蓝色荧光染料。和双链DNA结合后可以产生比DAPI自身强20多倍的荧光。和EB(ethidium bromide)相比，对双链DNA的染色灵敏度要高很多倍。
DAPI染色常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常用于普通的细胞核染色以及某些特定情况下的双链DNA染色。
DAPI的最大激发波长为340nm，最大发射波长为488nm；DAPI和双链DNA结合后最大激发波长为364nm，最大发射波长为454nm。
危险性质
DAPI对人体有一定刺激性，请注意适当防护。
荧光染料都存在淬灭的问题，建议染色后尽量当天完成检测。
为减缓荧光淬灭可以使用抗荧光淬灭封片液。抗荧光淬灭封片液可以向碧云天订购。了您的安全和健康，请穿实验服并戴一次性手套操作。
用途：用于细胞核染色时，推荐的DAPI工作浓度为0.5-10微克/毫升。
存储方式：-20℃避光保存，一年有效。
相关产品：
P1016 4×蛋白上样缓冲液（含β-巯基乙醇）
P1017 4×非变性蛋白上样缓冲液
A1030 10%过硫酸氨
P0012 10×丽春红染色液
P1300-500 考马斯亮蓝快速染色液
P1300 SDS-PAGE凝胶制备试剂盒
PR1700 预染次高分子量蛋白MARKER
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公司简介：
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英文名称: 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI)
说明：DNA链荧光染色。
分子式：C16H15N5 ·2HCl
分子量：350.25
CAS＃：28718-90-3
外观：黄色结晶性粉末
特性：纯度：≥90% (HPLC和TLC)
溶解性：溶于水，参考浓度20mg/ml，加热有助于溶解。
储存条件：−20℃避光保存
DAPI是一种可以穿透细胞膜的蓝色荧光染料。和双链DNA结合后可以产生比DAPI自身强20多倍的荧光。和EB（ethidium bromide）相比，对双链DNA的染色灵敏度要高很多倍。DAPI染色常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常用于普通的细胞核染色以及某些特定情况下的双链DNA染色。
化学性质
DAPI是一种可以穿透细胞膜的蓝色荧光染料。和双链DNA结合后可以产生比DAPI自身强20多倍的荧光。和EB(ethidium bromide)相比，对双链DNA的染色灵敏度要高很多倍。
DAPI染色常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常用于普通的细胞核染色以及某些特定情况下的双链DNA染色。
DAPI的最大激发波长为340nm，最大发射波长为488nm；DAPI和双链DNA结合后最大激发波长为364nm，最大发射波长为454nm。
危险性质
DAPI对人体有一定刺激性，请注意适当防护。
荧光染料都存在淬灭的问题，建议染色后尽量当天完成检测。
为减缓荧光淬灭可以使用抗荧光淬灭封片液。抗荧光淬灭封片液可以向碧云天订购。了您的安全和健康，请穿实验服并戴一次性手套操作。
用途：用于细胞核染色时，推荐的DAPI工作浓度为0.5-10微克/毫升。
存储方式：-20℃避光保存，一年有效。
相关产品：
P1016 4×蛋白上样缓冲液（含β-巯基乙醇）
P1017 4×非变性蛋白上样缓冲液
A1030 10%过硫酸氨
P0012 10×丽春红染色液
P1300-500 考马斯亮蓝快速染色液
P1300 SDS-PAGE凝胶制备试剂盒
PR1700 预染次高分子量蛋白MARKER
相关文献：
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《Exosomes from microRNA-199-3p-modified adipose-derived stem cells promote proliferation and migration of endothelial tip cells by downregulation of semaphorin 3A》 作者:Lingjuan Du, Guojian Li, Yong Yang, Guokai Yang, Jia Wan, Zhenhuan Ma, Yi Hou 期刊:International Journal of Clinical and Experimental Pathology 影响因子:0.205 PMID:
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《Multiwall carbon nanotubes/polycaprolactone composites for bone tissue engineering application》 作者:Lanlan Pan,Xibo Pei,Rui He,Qianbing Wan,Jian Wang 期刊:Colloids and Surfaces B: Biointerfaces 影响因子:3.554 PMID:22305638
《S‐Allylmercaptocysteine Attenuates Cisplatin‐Induced Nephrotoxicity through Suppression of Apoptosis, Oxidative Stress, and Inflammation》 作者:Xiaosong Zhu , Xiaoyan Jiang , Ang Li , Zhongxi Zhao OrcID and Siying Li 期刊:nutrients 影响因子:4.196 PMID:
《Ras-association domain family 1 (RASSF1A) gene regulates progression, migration and invasion of bladder cancer》 作者:Yuhai Bao,Xuefeng Liu,Yang Liu,Si Wang,Bin Wu 期刊:Surgical Oncology 影响因子:2.136 PMID:
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《Generation of bioartificial hearts using decellularized scaffolds and mixed cells》 作者:Cailing Tong, Cheng Li, Baiyi Xie, Minghui Li, Xianguo Li, Zhongquan QiEmail author and Junjie Xia 期刊:BioMedical Engineering OnLine 影响因子:2.013 PMID:31164131
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《Examination of Alzheimer's disease by a combination of electrostatic force and mechanical measurement》 作者:W. ZHAO W. CUI S. XU L.‐Z. CHEONG C. SHEN 期刊:Journal of Microscopy 影响因子:1.813 PMID:31038737
《microRNA-690 regulates induced pluripotent stem cells (iPSCs) differentiation into insulin-producing cells by targeting Sox9》 作者:Yang Xu, Yan Huang, Yibing Guo, Yicheng Xiong, Shajun Zhu, Liancheng Xu, Jingjing Lu, Xiaohong Li, Jian Wan, Yuhua Luand Zhiwei Wang 期刊:Stem Cell Research & Therapy 影响因子:4.627 PMID:30767782
《Effect of photobiomodulation on neural differentiation of human umbilical cord mesenchymal stem cells》 作者:Hongli Chen,Hongjun Wu,Huijuan Yin,Jinhai Wang,Huajiang Dong ,Qianqian Chen,Yingxin Li 期刊:Lasers in Medical Science 影响因子:2.076 PMID:30232645
《The Damaging Effects of Pedunsaponin A on Pomacea canaliculata Hemocytes》 作者:Chunping Yang , Tianxing Lv ,Bin Wang,Xiaoyan Qiu,Liya Luo ,Min Zhang , Guizhou Yue , Guangwei Qin , Deshan Xie and Huabao Chen 期刊:Toxins 影响因子:3.895 PMID:31277361
《ApoM-S1P Modulates Ox-LDL-Induced Inflammation Through the PI3K/Akt Signaling Pathway in HUVECs》 作者:Zhi Zheng,Yongzhi Zeng,Xiao Zhu,Ying Tan,Yi Li,Qian Li,Guanghui Yi 期刊:Inflammation 影响因子:2.939 PMID:30377890
《The expression of melatonin receptors MT1 and MT2 is regulated by E2 in sheep oviduct》 作者:Jun-jie Hu，Long-fei Xiao，Liang-li Song，Wen-bo Ge，Hong-wei Duan，Yuting Jiang 期刊:General and Comparative Endocrinology 影响因子:2.445 PMID:30849411
《The lncRNA SNHG16 affects prognosis in hepatocellular carcinoma by regulating p62 expression》 作者:Jian‐Hong Zhong , Xiao Xiang , Yan‐Yan Wang, Xu Liu, Lu‐Nan Qi , Cheng‐Piao Luo, Wen‐E Wei, Xue‐Mei You , Liang Ma , Bang‐De Xiang Le‐Qun Li 期刊:Journal of Cellular Physiology 影响因子:4.522 PMID:31256427
《lncRNA Gm10451 regulates PTIP to facilitate iPSCs-derived β-like cell differentiation by targeting miR-338-3p as a ceRNA》 作者:Yan Huang,Yang Xu, Yuhua Lu,Shajun Zhu,Yibing Guo,Cheng, Sun,Lianchen Xu ,Xiaolan Chen,Yahong Zhao,Bin Yu,Yumin Yang,Zhiwei Wang 期刊:Biomaterials 影响因子:10.273 PMID:31220795
《The role and mechanism of transforming growth factor beta 3 in human myocardial infarction‐induced myocardial fibrosis》 作者:Ke Xue , Jun Zhang , Cong Li , Jing Li , Cong Wang, Qingqing Zhang, Xianlu Chen , Xiaotang Yu , Lei Sun ,Xiao Yu 期刊:Journal of Cellular and Molecular Medicine 影响因子:4.658 PMID:30983140
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《Zoledronic acid inhibits osteoclast differentiation and function through the regulation of NF-κB and JNK signalling pathways》 作者:Xiao?Lin Huang， Lie?Yu Huang， Yu?Ting Cheng ，Fang Li ，Qian Zhou ，Chao Wu， Qian?Hui Shi， Zhi?Zhong Guan ，Jian Liao ，Wei Hong 期刊:International Journal of Molecular Medicine 影响因子:2.928 PMID:31173157
《Monosialoganglioside protects against bupivacaine-induced neurotoxicity caused by endoplasmic reticulum stress in rats》 作者:Benquan Liu,Jiemei Ji, Qing Feng, Xi Luo, Xiurong Yan, Yuxia Ni, Yajun He, Zhongxuan Mao and Jingchen Liu 期刊:Drug Design, Development and Therapy 影响因子:3.208 PMID:30858700
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《Halloysite nanotubes coated 3D printed PLA pattern for guiding human mesenchymal stem cells (hMSCs) orientation》 作者:Fan Wu,Jingqi Zheng,Zhixiong Li,Mingxian Liu 期刊:Chemical Engineering Journal 影响因子:8.355 PMID:
《CFLAR is a critical regulator of cerebral ischaemia-reperfusion injury through regulating inflammation and endoplasmic reticulum (ER) stress》 作者:Xiaohong Wang，Jun Zhao，Hongmei Guo，Qinqin Fan 期刊:Biomedicine & Pharmacotherapy 影响因子:3.743 PMID:
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文献和实验相关实验
1 ）核 DNA 和线粒体 DNA 1． 当细胞培养到约 107 细胞 /ml 时，离心（ 5 秒）收集细胞，再悬浮在 70 ％乙醇中。 2． 固定 5 分钟或 5 分钟以上，用水洗 2 次。 3． 将细胞悬浮在含有 50ng/ml 的 4 ， 6- 二脒基 -2 苯基吲哚的封固剂中。 1mg/ml 的 4 ， 6- 二脒基 -2 苯基吲哚母液可在－ 20 ℃下贮存。 4． 用紫外滤片观察。
酵母活体染色 1 ）核 DNA 和线粒体 DNA 1． 当细胞培养到约 107 细胞 /ml 时，离心（ 5 秒）收集细胞，再悬浮在 70 ％乙醇中。 2． 固定 5 分钟或 5 分钟以上，用水洗 2 次。 3． 将细胞悬浮在含有 50ng/ml 的 4 ， 6- 二脒基 -2 苯基吲哚的封固剂中。 1mg/ml 的 4 ， 6- 二脒基 -2 苯基吲哚母液可在－ 20 ℃下贮存
未消化完的组织片，离心去上清液后，加入冰冷的70％酒精固定，放入4℃冰箱备用。上机前染色。DNA特异荧光染料主要有:（1）碘化丙锭（PI）（2）溴化乙锭（EB）（3）4，6-二脒基-二苯基吲哚（DAPI）
技术资料暂无技术资料 索取技术资料
4',6-二脒基-2-苯基吲哚 -----DAPI---可用于细胞核染色的试剂
¥800
