Blank control:NIH/3T3.
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Isotype Control:Rabbit IgG(bs-0295P)
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Dilution: 1ug/Test;
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Primary Antibody (green line): Rabbit Anti- beta-Actin antibody (bs- 0061R)
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Dilution: 1ug/Test;
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Secondary Antibody : Goat anti-rabbit IgG-FITC
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Dilution: 1ug/Test.
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Protocol
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The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Isotype control antibody (orange line) was rabbit IgG . The secondary antibody(blue line) used for 40 min at room temperature.Acquisition of 20,000 events was performed._x000D_
- ￥264 - 1100
- Bioss
- bs-0295P
- 2026年10月10日
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10mg/50mg/100mg
| 规格： | 10mg | 产品价格： | ￥264.0 |
|---|---|---|---|
| 规格： | 50mg | 产品价格： | ￥660.0 |
| 规格： | 100mg | 产品价格： | ￥1100.0 |
| 产品编号 | bs-0295P |
| 英文名称 | Rabbit IgG |
| 中文名称 | 兔IgG（亲和层析纯化) |
| 英文别名 | Rabbit Immunoglobulin G; Normal Rabbit IgG; Native Rabbit IgG |
| 中文别名 | (亲和层析纯化) 兔IgG; 正常兔免疫球蛋白 |
| 性状 | Lyophilized |
| 储存液 | 0.01M PBS (pH7.4). |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
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【信息】Rabbit anti-GAPDH(SF-PA005)（兔抗 GAPDH 多克隆 IgG 抗体）招代理
Rabbit anti-GAPDH(SF-PA005)（兔抗 GAPDH 多克隆 IgG 抗体） 背景 甘油醛 -3- 磷 酸脱氢酶（ Glyceraldehyde 3-phosphate dehydrogenase，GAPDH）是糖酵解（glycolysis）过程中的关键酶，分子量为37kDa 左右。它催化甘油醛-3-磷酸的氧化磷酸化反应。GAPDH 作为看家基因 在同种细胞 或者组织中一般是恒定、高水平表达的，常用作 Western Blot 实验的内参照. 来源 兔抗
blots. In direct ELISAs, this antibody shows less than 2% cross-reactivity with human IgG and rabbit IgG. Optimal dilutions should be determined by each laboratory for each application. 谢谢!!!
到 PVDF 膜上，同时减少蛋白不必要降解，这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息：1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat
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Rabbit IgG(bs-0295P)-10mg/50mg/100mg
￥264 - 1100
