- ￥1350 - 3000
- Proteintech
- 68055-1-Ig
- 2026年05月28日
- IHC, RIP, Dot Blot, ELISA
- Mouse
- chemical compound, m6a
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- 文献和实验
- 技术资料
- 亚型：
IgG3
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
chemical compound, m6a
- 宿主：
Mouse
- 应用范围：
IHC, RIP, Dot Blot, ELISA
- 靶点：
m6A
- 抗体英文名：
m6A Monoclonal antibody
- 抗体名：
m6A Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive RIP detected in | RNA |
| Positive IHC detected in | mouse testis tissue, human lung cancer tissue, human breast cancer tissue, human colon cancer tissue, rat lymph node Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive ELISA detected in | m6A |
| Positive Dot Blot detected in | RNA |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| RIP | RIP : 5-10μg antibody/mg lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:2000-1:20000 |
| DOT BLOT | DOT BLOT : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68055-1-Ig targets m6A in WB, IHC, IF, IP, ChIP, RIP, Dot Blot, ELISA applications and shows reactivity with chemical compound, m6a samples.
| 经测试应用 | IHC, RIP, Dot Blot, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, ChIP, RIP, ELISA |
| 经测试反应性 | chemical compound, m6a |
| 文献引用反应性 | human, mouse, rat, pig, monkey |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG3 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | m6A |
| 别名 | 6-N-Methyladenoside, N6-methyladenosine |
| GenBank蛋白编号 | m6A |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2918796 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
m6A (N6-methyladenosine) is the most abundant internal modification in mammalian mRNA. This modification is installed by the m6A methyltransferases or termed "writers"such as METTL3 and METTL14, and can be reversed by demethylases that serve as "erasers" such as FTO and ALKBH5. The stability of m6A-modified mRNA is regulated by m6A reader protein YTHDFs, which recognizes m6A and reduces the stability of target transcripts. m6A modification and its regulatory proteins play critical roles in cancer pathogenesis and progression. m6A modification is also invovled in viruses life cycles, suggesting that drugs targets to m6A pathway could be used for antiviral thereapy.
Protocol for Dot Blot:
https://www.ptglab.com/protocol/68055-1-IgDotBlot.pdf
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文献和实验
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