BioDynami NGS DNA建库定制试剂盒——illu

BioDynami NGS DNA建库定制试剂盒——illumina平台

产品简介：

该试剂盒可用于构建高质量的二代测序文库。该试剂盒来自BioDynami的NGS DNA建库试剂盒。同样，该试剂盒需要DNA片段作为文库构建的输入，并与酶法和物理方法生成的DNA片段兼容。酶DNA碎片(DNA碎片和a -加尾酶混合Cat. # 40062)和物理DNA碎片(超声等)与定制试剂盒有很大的关联。

随着二代测序应用的扩展，对库准备的要求非常多样化，在某些情况下需要准备定制的库。为了方便DNA文库的定制，BioDynami制作了试剂盒来帮助科学家们完全定制他们自己的二代测序文库的形式。BioDynami的试剂盒允许科学家使用他们自己设计的适配器，PCR引物和PCR试剂。

在文库准备和后续PCR扩增步骤后，可使用标准珠纯化对构建的文库进行清洗。优化后的微珠纯化步骤不仅缩短了工作时间，还减少了所需微珠量的一半以上。

The kit was developed for construction of high quality libraries for next generation sequencing. The kit is derived from our NGS DNA Library Prep Kit.  Similarly, the kit needs DNA fragments as input for library construction, and is compatible with DNA fragments generated from enzymatic methods and physical methods. Both enzymatic DNA fragmentation (DNA Fragmentation & A-tailing Enzyme Mix Cat.# 40062) and physical DNA fragmentation (sonication etc) have great outcome with the customization kit.

With the expansion of the NGS applications, requirements for library preparation are very diverse and in some cases more customized library prep is needed. To facilitate the DNA library prep customization, we made the kit to help the scientists so they can totally customize the form of their own NGS libraries. Our kit allows the scientists to use their own designed adaptors, PCR primers, and PCR reagents.

Standard beads purification can be used to clean the constructed libraries after the library prep and subsequent PCR amplification step. The optimized beads purification step not only reduces the working time, but also decreases more than half of the beads amount needed.

 

特征

        快
        动手时间：~4 min
        总时间：<1 小时
        简单的工作流程：文库构建的两个反应步骤
        更高的库转换效率
        起始 DNA 量为 100 ng 至 1 ug
Features

• • Fast
    • Hands-on time: ~4 min
    • Total time: <1 hrs
  • Simple workflow: Two reaction steps for library construction
  • Higher library conversion efficiency
  • Input DNA amount from 100 ng to 1 ug

 

产品订购：

产品名称	货号	产品规格
NGS DNA Library Prep Customization Kit(illumina)	30001S	24 Reactions
NGS DNA Library Prep Customization Kit(illumina)	30001L	48 Reactions