BioDynami PCR-Free NGS DNA文库制备

产品简介：

BioDynami PCR-Free NGS DNA建库试剂盒，用于二代测序的DNA文库(illumina平台)。该试剂盒有效地将3 ' -dT-tailed库适配器添加到DNA片段的两端。该试剂盒使用双链DNA片段(钝性和/或粘性)作为NGS文库构建的输入DNA，并与酶促法(BioDynami DNA片段酶等)和物理法(超声、雾化等)产生的DNA片段兼容。

 

BioDynami PCR-Free NGS DNA建库试剂盒工作流程

PCR扩增是二代测序文库制备的标准步骤。PCR用于扩增完全连接的DNA片段并为文库添加index信息。index是为了汇集库样本，以减少测序成本。然而，PCR在一些GC含量和二级结构极高的DNA区域引入了不均匀扩增的DNA。这种偏倚可能导致这些地区的测序覆盖率非常低。这些区域的DNA测序仍然是一个巨大的挑战。PCR-Free NGS DNA建库可以减少文库偏倚并最小化测序缺口。来自PCR-free文库样本的测序数据甚至具有基因组覆盖，几乎没有空白区和更好的GC丰富区域深度。BioDynami PCR-Free NGS DNA建库试剂盒可以在传统上比较困难的区域提供最佳覆盖，例如高GC含量区域，低GC含量区域和重复序列区域。

酶剪切
• DNA 剪切和文库制备：BioDynami NGS DNA 片段化和文库制备试剂盒 机械剪切
• DNA 剪切：Covaris 超声处理
• 文库制备：BioDynami NGS DNA 文库制备试剂盒

试剂盒有两种index类型：

Non-index：库没有索引。

Index：每个库包含一个i5索引和一个i7索引。库复用可多达96个样本。

产品优势：

  ★ 总时间：1小时；

  ★ 操作时间：5分钟；

  ★ 简单的程序：准备使用的主混合和较少的反应试剂盒；

  ★ DNA输入量从100微克到1微克。

PCR-Free NGS DNA Library Prep Kit

The PCR-free NGS DNA Library Prep Kit was developed for construction of DNA libraries for next generation sequencing (illumina platform). The kit adds 3′-dT-tailed library adapters to both ends of DNA fragments efficiently. The kit uses double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library construction, and is compatible with DNA fragments generated from both enzymatic methods (BioDynami DNA fragmentation enzymes etc.) and physical methods (sonication, nebulization etc.).

PCR-Free NGS DNA Library Prep Kit Workflow

PCR amplification is a standard step for library preparation of Next-Generation Sequencing. The PCR is used to amplify fully ligated DNA fragments and to add index information to the libraries. The indexing is for the pooling of the library samples in an effort to reduce the sequencing cost.

However, PCR introduces uneven amplification of DNA in some DNA regions with extreme GC-contents and secondary structures. This bias can cause very low sequencing coverage in such regions. DNA sequencing in these regions is still a huge challenge.

PCR-free library prep can reduce library bias and minimize sequencing gaps. The sequencing data from PCR-free library samples have even genomic coverage with few gaps and better depth in GC-rich regions. Our PCR-free NGS kit offers optimal coverage in the regions that are traditionally difficult, such as high-GC content regions, low-GC content regions, and repetitive sequence regions.

Two index types are available for the kit:

Non-index: Libraries do not have index.

Index: Each library contains one i5 index and one i7 index.  Library multiplexing up to 96 samples is possible. List of indexes can be downloaded Here.

Kit advantages:

• • • Total time: 1 hr
    • Hands-on time: 5 min
    • Easy procedure: Ready-to-use master mix & Less reaction components
    • Input DNA amount from 100 ng to 1 ug