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- 详细信息
- 用户评价
- 文献和实验
- 技术资料
- 保存条件：
2-8℃避光保存6个月。
- 保质期：
6个月
- 英文名：
ECL luminescence reagent,ECL发光液,HRP化学发光底物
- 库存：
现货
- 供应商：
爱必信(上海)生物科技有限公司
- 规格：
2*250mL/2*50mL/2*25mL
| 规格： | 2*250mL | 产品价格： | ￥1155.0 |
|---|---|---|---|
| 规格： | 2*50mL | 产品价格： | ￥368.0 |
| 规格： | 2*25mL | 产品价格： | ￥210.0 |
| 描述 | Western blot底物发光检测试剂可由标记于二抗上的辣根过氧化酶催化，产生化学发光反应，可以灵敏地检测出目的蛋白的存在。ECL底物化学发光检测试剂盒基于新一代增强型化学发光底物研制而成，并对成份做了优化。产品背景低，稳定性好。它由辣根过氧化物酶(HRP)催化发生化学反应，发出荧光，可对X光胶片曝光，也可直接进行luminometer检测或者荧光CCD扫描。 |
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| 产品组分 | A液；B液 | ||||||||||||||||||||||||||||||||||||
| 技术指标 |
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| 操作步骤 | 1、按常规Western blot操作，二抗孵育后，进行最后一次洗涤时，根据膜的大小，按每10 cm2 膜混合0.5ml溶液A和0.5ml溶液B混匀，配制成发光检测工作液。 2、用平头镊子将膜取出，膜的下缘轻轻接触吸水纸，以去除膜上多余的液体。膜的蛋白面朝上，置于洁净保鲜膜（某些市售保鲜膜包裹印迹膜时可能会淬灭荧光，应选择高质量保鲜膜）上。用吸管将配制的发光检测液转移到蛋白膜上，使其均匀覆盖，室温孵育2分钟。 3、用平头镊夹持蛋白膜，膜的下缘轻轻接触吸水纸，以去除膜上多余的液体。膜的蛋白面朝上，包裹于洁净保鲜膜内。轻轻赶出其间的气泡，固定在X片暗盒内。 4、在暗室中取一张X片置于包裹的膜上，合上暗盒，曝光30秒至1分钟。立即显影定影，根据其曝光强度，缩短或延长下一张X片的曝光时间（对微弱信号，曝光时间可延长至数小时）。 |
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| 操作说明 | 1、ECL A液和B液在吸取过程中必须要更换枪头，A液和B液相互污染后会导致 A液或B液逐渐失效，影响后续的使用效果。 2、各溶液使用后，请盖紧瓶盖，以防失效。特别是B液，含有氧化剂，比较容易 被还原而失效。 3、ECL的荧光持续时间很长，但开始反应后的30分钟内荧光更强一些，随后荧光会逐渐减弱，因此请注意充分利用这荧光较强的30分钟进行压片。 4、ECL A液和B液均对人体有害，操作时请注意适当防护。 为了您的安全和健康，请穿实验服并戴一次性手套操作。 |
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| 基本信息 | |||||||||||||||||||||||||||||||||||||
| 别名 | ECL发光液,HRP化学发光底物 | ||||||||||||||||||||||||||||||||||||
| 储存/保存方法 | 2-8℃避光保存6个月。 | ||||||||||||||||||||||||||||||||||||
实验结果图：
Western blot analysis of Rock-1 expression in Hep G2 (A) and NIH/3T3 (B) whole cell lysates.
Western blot analysis of NFATc3 isoform expression in Ramos whole cell lysate.
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文献和实验该产品被引用文献或证书
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[2]Comprehensive proteomic and phosphoproteomic reveal that Microcystin-LR contributed to the malignant progression of gastric cancer by estrogenic potency
[3]Demethylation of miR-299-5p by aerobic exercise relieves insulin resistance in the vascular endothelium by repressing resistin
[4]AhR regulates VEGF expression by promoting STAT1 transcriptional activity, thereby affecting endothelial angiogenesis in acute limb ischemia
[5]Protective Effects of Berberine against β-Amyloid-Induced Neurotoxicity in HT22 Cells via the Nrf2/HO‐1 Pathway
[6]RUNX2 interacts with SCD1 and activates Wnt/β-catenin signaling pathway to promote the progression of clear cell renal cell carcinoma
[7]Overexpression of RAB34 associates with tumor aggressiveness and immune infiltration in glioma
[8]Sasanquasaponin inhibited epithelial to mesenchymal transition in prostate cancer by regulating the PI3K/Akt/mTOR and Smad pathways
[9]Differential Expression of α-Enolase in Clinical Gastric Tissues and Cultured Normal/Cancer Cells in Response to Helicobacter pylori Infection and cagA Transfection
[10]Autologous Bioactive Compound Concentrated Growth Factor Ameliorates Fistula Healing of Anal Fistula in a Pig Model and Promotes Proliferation and Migration of Human Skin Fibroblasts via Regulating the MEK/ERK Pathway
[11]A Mouse Model of Damp-Heat Syndrome in Traditional Chinese Medicine and Its Impact on Pancreatic Tumor Growth
more...
研选
ECL化学发光检测试剂盒,ECL发光液
￥210 - 1155
