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- 文献和实验
- 技术资料
- 保存条件：
2-8℃
- 保质期：
4年
- 英文名：
ctDNA
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- CAS号：
73049-39-5
- 规格：
50mg
| 有效期 | 4年 |
|---|---|
| 来源 | from calf thymus |
| 别名 | ctDNA;胸腺核酸钠盐;脱氧核糖核酸钠盐（来源于小牛胸腺） |
| 英文名称 | Deoxyribonucleic acid sodium salt from calf thymus（DNA(calf thymus)） |
| CAS | 73049-39-5 |
| 储存条件 | 2-8℃ |
| 外观（性状） | 纤维状的 |
| 单位 | 支 |
小牛胸腺DNA(CT-DNA)是一种天然的DNA广泛用于研究DNA绑定抗癌剂和DNA绑定代理,调节DNA结构和功能。
小牛胸腺DNA的DNA也被运用于物理化学研究行为的解决方案。 溶解性：溶于水，参考浓度1mg/ml，4℃条件下轻轻摇晃过夜为最佳方法，但不可剧烈震荡。
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分离出DNA。 小牛胸腺、猪脾、鱼精子和植物种子的胚胎等生物材料，细胞核含量比例大，因而含有丰富的DNA，是提取DNA的好材料。本实验以小牛胸腺或猪脾为材料。小牛胸腺不仅DNA含最高，而且其脱氧核糖核酸酶（DNase）的活性较低，在制备过程中由此酶所引起的DNA降解损失也较小。为了防止DNase的作用，在用于提取的缓冲液中均含有1mmol/L的EDTA（乙二胺四乙酸）螫合剂，以除去保持 DNase活性所必须的Mg++离子。为防止DNA的变性和降解，操作要尽可能在低温下进行。 本实验通过匀浆
问： 实验室购买的是西格玛产的小牛胸腺DNA，说明书上说保存温度是2~8℃，而由于我们的粗心，我们将其储存在零下20℃的低温储藏箱内，而我们将其配成溶液后，接下来又存储在0~4℃的普通冰箱里。我们用这个保存条件下的DNA做精密度较高的电化学实验，结果发现毫无效果，有指导老师告诉我有可能因为第一步零下20度太低，“冻死了”，请各位虫友支支招，到底应该在什么温度下保存比较好，或者告诉我小牛胸腺
机能，以微量存在于血中，以环核苷酸（ cAMP）作为第二信使，可视为胸腺激素（ thymin）。其中研究最多的是胸腺素（ thy-mosin）。胸腺素为怀特（ White）和戈尔茨坦（ Goldstein）从小牛胸腺中提取出来的、分子量为 12000道尔顿的蛋白质。能使免疫缺陷病人的 T细胞机能得到恢复，可诱导无胸腺及去胸腺小鼠的 T细胞机能，并可增加小鼠胸腺细胞中的环鸟苷酸。此外，胸腺激素Ⅰ，也是从小牛胸腺中提取出来的多肽，以后进一步提纯成胸腺激素Ⅱ，亦有诱导 T细胞的机能。此激素存在于胸腺
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