- ￥1200 - 7200
- Proteintech
- HRP-60004
- 2025年10月13日
- WB
- Mouse
- human, mouse, rat, zebrafish
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG2b
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
HRP
- 适应物种：
human, mouse, rat, zebrafish
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB
- 靶点：
GAPDH
- 抗体英文名：
HRP-conjugated GAPDH Monoclonal antibody
- 抗体名：
HRP-conjugated GAPDH Monoclonal antibody
- 规格：
100ul/500ul/1ml
| 规格： | 100ul | 产品价格： | ￥1200.0 |
|---|---|---|---|
| 规格： | 500ul | 产品价格： | ￥4800.0 |
| 规格： | 1ml | 产品价格： | ￥7200.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, mouse brain tissue, HepG2 cells, HEK-293 cells, mouse lung tissue, mouse liver tissue, mouse kidney tissue, mouse heart tissue, mouse thymus tissue, mouse spleen tissue. mouse skin tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
HRP-60004 targets GAPDH in WB, IHC, IF, IP, CoIP, RIP applications and shows reactivity with human, mouse, rat, zebrafish samples.
| 经测试应用 | WB |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, RIP |
| 经测试反应性 | human, mouse, rat, zebrafish |
| 文献引用反应性 | human, mouse, rat, pig, rabbit, monkey, chicken, hamster, sheep, honey bees |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | glyceraldehyde-3-phosphate dehydrogenase |
| 别名 | 1E6D9 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC004109 |
| 基因名称 | GAPDH |
| Gene ID (NCBI) | 2597 |
| RRID | AB_2737588 |
| 偶联类型 | HRP |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P04406 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Nature DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25.Authors - Linbin Yang |
| human | WB | Mol Cancer Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancerAuthors - Rashmi Bharti |
| human | WB | Nat Biotechnol Direct targeting of amplified gene loci for proapoptotic anticancer therapy.Authors - Meetu Kaushik Tiwari |
| human | WB | Cell Programmable RNA-Guided RNA Effector Proteins Built from Human Parts.Authors - Simone Rauch |
| Mouse | WB | Cell Complement Signals Determine Opposite Effects of B Cells in Chemotherapy-Induced Immunity.Authors - Yiwen Lu |
| human | WB | Cell Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages.Authors - Shicheng Su |
底物范围较广，包括二氨基联苯胺 (DAB)、氯萘酚和氨乙基咔唑等。 二氨基联苯胺/金属盐 DAB 是辣根过氧化物酶最敏感、常用的底物。它产生强烈的棕色产物，在水和醇中不溶解。多数情况下，推荐在 DAB 中加入不同的金属离子。当加入金属盐如钴、镍至底物液中，辣根过氧化物酶可以更加敏感。此类反应产物呈暗蓝灰色至黑色，且在水及醇中稳定。DAB/金属盐染色应用的组织染色范围较广。 1. 需要的溶液和特殊设备 DAB，0.05 mol/L Tris 缓冲液 (pH7.6), 0.3％ (W
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显色（HRP酶） 1．增强化学发光法(ECL) Ecl 显色原理：氨基苯二酰肼类主要是鲁米诺及异鲁米诺衍生物，是最常用的一类化学发光剂。鲁米诺（luminol，5＇-氨基-2，3-二氢-1，4-酞嗪二酮）的分子结构及化学反应式如下： 鲁米诺在免疫测定中既可用作标记物，也可用作过氧化物酶的底物。在Ecl底物中，含有H2O2和鲁米诺，在HRP（辣根过氧化物酶）的作用下，发出荧光来。 试验步骤： 1）将两种显色底物1：1等体积混合（一般各1ml/membrane）。
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