p38 MAPK Antibody

价格询价

品牌Cell Signaling Technology
货号
9212
产地USA
更新日期2025年09月06日

应用范围W, IHC-P, IF-IC, F
宿主Rabbit
适应物种H,M,R,Mk,GP,C

美国CST中国公司(丁香通商铺)

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详细信息
询价记录
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抗体英文名：
p38 MAPK Antibody
抗原：
synthetic peptide corresponding to the sequence of human p38 MAPK
应用范围：
W, IHC-P, IF-IC, F
宿主：
Rabbit
适应物种：
H,M,R,Mk,GP,C
库存：
大量
供应商：
CST
级别：
详见MSDS文件
保质期：
详见说明书
是否单克隆：
2
保存条件：
-20°c
规格：
40 ul (8 western blots)/200 ul (20 western blots)/600 ul (60 western blots)/carrier free & custom formulation / quantity

规格：		产品价格：	￥请询价
规格：	40 ul (8 western blots)	产品价格：	￥请询价
规格：	200 ul (20 western blots)	产品价格：	￥请询价
规格：	600 ul (60 western blots)	产品价格：	￥请询价
规格：	carrier free & custom formulation / quantity	产品价格：	￥请询价

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken GP=Guinea Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IHC-P IF-IC F	H M R Mk GP (C)	Endogenous	43	Rabbit

Protocols	9212: Flow , IHC / Paraffin , Immunofluorescence , Western Blotting
Specificity / Sensitivity	p38 MAP Kinase Antibody detects endogenous levels of total p38α, -β or -γ MAPK protein. This antibody does not recognize p38δ, JNK/SAPK or p44/42 MAPK.
Source / Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human p38 MAPK. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence^® Control siRNA (Unconjugated) #6568 (-), SignalSilence^® p38 MAPK siRNA I #6564 (+) or SignalSilence^® p38 MAPK siRNA II (+), using p38 MAPK Antibody #9212 and α-Tubulin (11H10) Rabbit mAb #2125. The p38 MAPK antibody confirms silencing of p38 MAPK expression while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of p38 MAPK siRNA. Western Blotting Western blot analysis of extracts from C6 cells, untreated or anisomycin-treated, and NIH/3T3 cells, untreated or UV-treated, using phospho-p38 MAPK (Thr180/Tyr182) Antibody #9211 (upper) or p38 MAPK Antibody (lower). Western Blotting Western blot analysis of extracts from NIH/3T3 cells, untreated or UV-treated for the indicated times, using Phospho-p38 MAPK (Thr180/Tyr182) Antibody #9211 (upper) or p38 MAPK Antibody (lower). IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using p38 MAPK Antibody. Flow Cytometry Flow cytometric analysis of Jurkat cells, using p38 MAPK Antibody (blue) compared to a nonspecific negative control antibody (red). IF-IC Confocal immunofluorescent images of HeLa cells-/+ UV light labeled with #9212 p38 MAPK (green) exhibiting cytoplasmic localization in untreated cells (left) and nuclear localization in treated cells (right). Red = Actin filaments (phalloidin).
Background	p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10). Rouse, J. et al. (1994) Cell 78, 1027-1037. Han, J. et al. (1994) Science 265, 808-811. Lee, J.C. et al. (1994) Nature 372, 739-746. Freshney, N.W. et al. (1994) Cell 78, 1039-1049. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426. Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534. Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30. Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038. Cuenda, A. et al. (1995) FEBS Lett 364, 229-33. Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.
Application References	Miyaji, M. et al. (2009) Proc Natl Acad Sci U S A 106, 14502-7. Applications: Western Blotting Hsu, J.T. et al. (2007) Am J Physiol Heart Circ Physiol 292, H2982-7. Applications: Western Blotting Kojima, R. et al. (2004) Biochem J 380, 783-94. Applications: Western Blotting Matsumoto, M. et al. (2004) J Biol Chem 279, 45969-79. Applications: IF-IC (In Cells) Matsumoto, M. et al. (2000) Involvement of p38 mitogen-activated protein kinase signaling pathway in osteoclastogenesis mediated by receptor activator of NF-kappa B ligand (RANKL). J. Biol. Chem. 275, 31155-31161. Applications: Western Blotting Chen, C. H. et al. (2000) Cyclic AMP activates p38 mitogen-activated protein kinase in Th2 cells: phosphorylation of GATA-3 and stimulation of Th2 cytokine gene expression. J. Immunol. 165, 5597-5605. Applications: Western Blotting Mudgett, J. S. et al. (2000) Essential role for p38alpha mitogen-activated protein kinase in placental angiogenesis. Proc. Natl. Acad. Sci. USA 97, 10454-10459. Applications: IHC-P (paraffin) Western Blotting Rossi, A. et al. (2000) Anti-inflammatory cyclopentenone prostaglandins are direct inhibitors of IkappaB kinase. Nature 403, 103-108. Applications: Western Blotting Zhuang, S. et al. (2000) p38 mitogen-activated protein kinase mediates bid cleavage, mitochondrial dysfunction, and caspase-3 activation during apoptosis induced by singlet oxygen but not by hydrogen peroxide. J. Biol. Chem. 275, 25939-25948. Applications: Western Blotting Rohani, M.G. et al. (2010) BMC Immunol 11, 53. Applications: Western Blotting Gehani, S.S. et al. (2010) Mol Cell 39, 886-900. Applications: Western Blotting Lee, W.H. et al. (2011) J Biol Chem 286, 21577-87. Applications: Western Blotting Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products	8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit) 8112 SignalStain^® Antibody Diluent 9913 Phospho-p38 MAPK Pathway Sampler Kit 9213 p38 MAPK Control Cell Extracts 4511 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP^® Rabbit mAb 9215 Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb 2371 p38α MAPK (7D6) Rabbit mAb 9217 p38α MAPK (5F11) Mouse mAb 9228 p38α MAPK (L53F8) Mouse mAb 2339 p38β MAPK (C28C2) Rabbit mAb 2308 p38δ MAPK (10A8) Rabbit mAb 9216 Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb 4631 Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb 2307 p38γ MAPK Antibody 9211 Phospho-p38 MAPK (Thr180/Tyr182) Antibody 9218 p38α MAPK Antibody 9214 p38δ MAPK Antibody 9210 PhosphoPlus^® p38 MAPK (Thr180/Tyr182) Antibody Kit 9219 Phospho-p38 MAPK (Thr180/Tyr182) Mouse mAb (Sepharose Bead Conjugate) 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody 7074 Anti-rabbit IgG, HRP-linked Antibody 7720 Prestained Protein Marker, Broad Range (Premixed Format) 7727 Biotinylated Protein Ladder Detection Pack 7003 20X LumiGLO^® Reagent and 20X Peroxide For Research Use Only. Not For Use In Diagnostic Procedures. * Offer is for U.S. customers only. There is a limit of one (1) free trial size of product 8690 per order, subject to availability. Offer is good on orders placed March 29, 2012 through June 29, 2012.