Phospho-RSK2 (Tyr529) Antibody

9324:

Western Blotting

Phospho-RSK2 (Tyr529) Antibody detects endogenous RSK2 protein only when phosphorylated at Tyr529. This antibody cross-reacts with some tyrosine phosphorylated proteins including EGFR.

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr529 of human RSK2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from untreated or FGF-treated SK-N-MC cells, using Phospho-RSK2 (Tyr529) Antibody (upper) or RSK2 Antibody #9340 (lower).

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).

Phosphorylation of RSK2 on Tyr529 was identified at Cell Signaling Technology (CST) using PhosphoScan^® , our LC-MS/MS platform for phosphorylation site discovery. This study identifies RSK2 as a key substrate of FGFR3 in human t(4;14)-positive multiple myeloma (MM) cells. Constitutively active FGFR3 directly phosphorylates RSK2 on Tyr529, which primes RSK2 for activation by ERK1/2 (4). RSK2 can also be phosphorylated at Tyr529 by Src family tyrosine kinases Src and Fyn in response to EGF stimulation (5). Mutations in the RSK2 gene are associated with Coffin-Lowry syndrome (CLS), an X-linked disorder characterized by mental retardation and the presence of characteristic facial anomalies (6).

1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
4. Kang, S. et al. (2007) Cancer Cell 12, 201-14.
5. Kang, S. et al. (2007) J Biol Chem Submitted for Publication
6. Delaunoy, J.P. et al. (2006) Clin Genet 70, 161-6.

• Kang, S. et al. (2007) Cancer Cell 12, 201-14. Applications: Western Blotting
• Kang, S. et al. (2007) J Biol Chem Submitted for publication. Applications: Western Blotting

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• 9326 p90RSK Antibody Sampler Kit
• 3556 Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb
• 9335 Phospho-p90RSK (Ser380) (9D9) Rabbit mAb
• 9355 RSK1/RSK2/RSK3 (32D7) Rabbit mAb
• 9333 RSK1 Antibody
• 9340 RSK2 Antibody
• 9343 RSK3 Antibody
• 9341 Phospho-p90RSK (Ser380) Antibody
• 9344 Phospho-p90RSK (Thr359/Ser363) Antibody
• 9346 Phospho-p90RSK (Thr573) Antibody
• 9347 RSK1/RSK2/RSK3 Antibody
• 9348 Phospho-RSK3 (Thr356/Ser360) Antibody
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.