Bax Antibody

2772:

Immunoprecipitation , Western Blotting

Bax Antibody detects endogenous levels of total Bax protein. The antibody does not cross-react with other Bcl-2 family members.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal residues of human Bax. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from 293 (human), COS (monkey), L929 (mouse) and PC12 (rat) cells, using Bax Antibody.

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence^® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence^® Bax siRNA I (+) or SignalSilence^® Bax siRNA II (+), using Bax Antibody and α-Tubulin (11H10) Rabbit mAb #2125. The Bax Antibody confirms silencing of Bax expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Bax siRNA.

IP

Immunoprecipitation of Bax from Jurkat cell extract, using Bax Antibody. Lane 1 is the lysate control; lane 2 is antibody alone; lane 3 is antibody plus lysate.

Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis (3,4).

1. Wei, M.C. et al. (2001) Science 292, 727-730.
2. Jurgensmeier, J.M. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 4997-5002.
3. Narita, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 14681-14686.
4. Marzo, I. et al. (1998) Science 281, 2027-2031.

• Xie, S. et al. (2001) Reactive oxygen species-induced phosphorylation of p53 on serine 20 is mediated in part by Polo-like kinase-3. J. Biol. Chem. 276 (39), 36194-36199. Applications: Western Blotting
• Yusta, B. et al. (2002) Glucagon-like peptide-2 receptor activation engages Bad and glycogen synthase kinase-3 in a protein kinase A-dependent manner and prevents apoptosis following inhibition of phosphatidylinositol 3-kinase. J. Biol. Chem. 277 (28), 24896-24906. Applications: Western Blotting
• Leu, J. I. et al. (2004) Mitochondrial p53 activates Bak and causes disruption of a Bak-Mcl1 complex. Nat. Cell. Biol. 6, 443-450. Applications: IP Western Blotting
• Cheong, J. W. et al. (2003) Induction of apoptosis by apicidin, a histone deacetylase inhibitor, via the activation of mitochondria-dependent caspase cascades in human Bcr-Abl-positive leukemia cells. Clin. Cancer Res. 9, 5018-5027. Applications: Western Blotting
• Rusinol, A. E. et al. (2004) AKT/protein kinase B regulation of BCL family members during oxysterol-induced apoptosis. J. Biol. Chem. 279, 1392-1399. Applications: Western Blotting
• Widenmaier, S.B. et al. (2009) J Biol Chem 284, 30372-82. Applications: Western Blotting
• Ikner, A. and Ashkenazi, A. (2011) J Biol Chem 286, 21546-54. Applications: Western Blotting
• Pritchard, J.R. et al. (2011) Cancer Res 71, 5850-8. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 6321 SignalSilence^® Bax siRNA I
• 2762 Bcl-xL Antibody
• 4592 Bik Antibody
• 9292 Bad Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.