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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
hnRNP K (R332) Antibody
- 抗原:
synthetic peptide corresponding to residues surrounding Arg332 of human hnRNP K protein
- 应用范围:
W, IP, IF-IC, F
- 宿主:
Rabbit
- 供应商:
CST
- 适应物种:
H,M,R,Mk
- 库存:
大量
- 保质期:
详见说明书
- 级别:
详见MSDS文件
- 是否单克隆:
2
- 保存条件:
-20°c
- 规格:
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格: | 产品价格: | ¥请询价 | |
|---|---|---|---|
| 规格: | 100 ul (10 western blots) | 产品价格: | ¥请询价 |
| 规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC F | H M R Mk | Endogenous | 58-62 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | hnRNP K (R332) Antibody detects endogenous level of total hnRNP K protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg332 of human hnRNP K protein. Antibodies were purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from HeLa and Hep G2 cells using hnRNP K (R332) Antibody. Flow Cytometry
Flow cytometric analysis of HeLa cells using hnRNP K (R332) Antibody (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or UV-treated (right), using hnRNP K (R332) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). |
| Background | Heterogeneous nuclear ribonucleoprotein K (hnRNP K) belongs to a family of RNA binding multiprotein complexes (hnRNP proteins) that facilitate pre-mRNA processing and transport of mRNA from the nucleus to cytoplasm (1-3). hnRNP K contains three unique structural motifs termed KH domains that bind poly(C) DNA and RNA sequences (4,5). Intricate architecture enables hnRNP K to facilitate mRNA biosynthesis (6), transcriptional regulation (7), and signal transduction. Research studies have shown that cytoplasmic hnRNP K expression is increased in oral squamous cell carcinoma and pancreatic cancer, and may be a potential prognostic factor (8,9). hnRNP K coordinates with p53 to regulate its target gene transcription in response to DNA damage. Proteasome degradation of hnRNP K is mediated by E3 ligase MDM2 (10). The interaction between hnRNP K and c-Src leads to hnRNP K phosphorylation, which allows for hnRNP K activation of silenced mRNA translation (11).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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