Pyruvate Dehydrogenase (C54G1)

Rabbit mAb

价格询价

品牌Cell Signaling Technology
货号
3205
产地USA
更新日期2026年05月28日

应用范围W, IHC-P
适应物种H,M,R,Mk

美国CST中国公司(丁香通商铺)

品牌商

13 年钻石会员

详细信息
询价记录
文献和实验
技术资料

抗体英文名：
Pyruvate Dehydrogenase (C54G1) Rabbit mAb
抗原：
synthetic peptide corresponding to the sequence of human pyruvate dehydrogenase
应用范围：
W, IHC-P
级别：
详见MSDS文件
库存：
大量
保质期：
详见说明书
适应物种：
H,M,R,Mk
供应商：
CST
是否单克隆：
1
保存条件：
-20°c
规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity

规格：		产品价格：	￥请询价
规格：	100 ul (10 western blots)	产品价格：	￥请询价
规格：	carrier free & custom formulation / quantity	产品价格：	￥请询价

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IHC-P	H M R Mk	Endogenous	43	Rabbit IgG

Protocols	3205: IHC / Paraffin , Western Blotting
Specificity / Sensitivity	Pyruvate Dehydrogenase (C54G1) Rabbit mAb detects endogenous levels of total pyruvate dehydrogenase α1 subunit.
Source / Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human pyruvate dehydrogenase. Western Blotting Western blot analysis of extracts from various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb. Western Blotting Western blot analysis of various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Pyruvate Dehydrogenase (C54G1) Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Pyruvate Dehydrogenase (C54G1) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Background	The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO₂ in the presence of NAD⁺ . Acetyl-CoA then goes into the citric acid cycle where it reacts with oxaloacetate to form citrate. Acetyl-CoA is also used for fatty acid and cholesterol biosynthesis. The reaction of oxidative decarboxylation of pyruvate therefore serves as a critical link between glycolysis and the citric acid cycle and lipid metabolism. In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix (1). This complex is comprised of three enzymes: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3). Pyruvate dehydrogenase (E1) consists of two subunits: α and β. This enzyme catalyzes the removal of CO₂ from pyruvate. Mutations in the α subunits of pyruvate dehydrogenase (E1) lead to congenital defects that are usually associated with lactic acidosis, neurodegeneration and early death (2). Strumiło, S. (2005) Acta Biochim Pol 52, 759-64. Stacpoole, P.W. et al. (2003) Curr Gene Ther 3, 239-45.
Application References	Zhang, C. et al. (2011) Proc Natl Acad Sci U S A 108, 16259-64. Applications: Western Blotting Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
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