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        求救引物设计

        丁香园论坛

        1511
        1: AF075241. Sus scrofa prepro...[gi:3328338] Links

        LOCUS AF075241 451 bp mRNA linear MAM 17-MAY-2000
        DEFINITION Sus scrofa prepro-orexin mRNA, complete cds.
        ACCESSION AF075241
        VERSION AF075241.1 GI:3328338
        KEYWORDS .
        SOURCE Sus scrofa (pig)
        ORGANISM Sus scrofa
        Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
        Mammalia; Eutheria; Cetartiodactyla; Suina; Suidae; Sus.
        REFERENCE 1 (bases 1 to 451)
        AUTHORS Dyer,C.J., Touchette,K.J., Carroll,J.A., Allee,G.L. and
        Matteri,R.L.
        TITLE Cloning of porcine prepro-orexin cDNA and effects of an
        intramuscular injection of synthetic porcine orexin-B on feed
        intake in young pigs
        JOURNAL Domest. Anim. Endocrinol. 16 (3), 145-148 (1999)
        MEDLINE 99275384
        PUBMED 10343916
        REFERENCE 2 (bases 1 to 451)
        AUTHORS Matteri,R.L. and Dyer,C.J.
        TITLE Direct Submission
        JOURNAL Submitted (29-JUN-1998) Agricultural Research Service, USDA, Room
        S-143 ASRC, University of Missouri, Columbia, MO 65211, USA
        FEATURES Location/Qualifiers
        source 1..451
        /organism="Sus scrofa"
        /mol_type="mRNA"
        /db_xref="taxon:9823"
        /tissue_type="hypothalamus"
        /dev_stage="neonate"
        CDS 1..396
        /function="neuropeptide precursor; involved in appetite
        control"
        /codon_start=1
        /product="prepro-orexin"
        /protein_id="AAC26827.1"
        /db_xref="GI:3328339"
        /translation="MNPPFAKVSWATVTLLLLLLLLPPAVLSPGAAAQPLPDCCRQKT
        CSCRLYELLHGAGNHAAGILTLGKRRPGPPGLQGRLQRLLQASGNHAAGILTMGRRAG
        AEPAPRLCPGRRCLAAAASSVAPGGRSGI"
        sig_peptide 1..99
        mat_peptide 100..198
        /product="orexin-A"
        mat_peptide 208..291
        /product="orexin-B"
        misc_feature 1..393
        /note="encodes prepro-orexin"
        misc_feature 100..393
        /note="encodes pro-orexin
        1 atgaatcctc cttttgcaaa ggtctcctgg gccaccgtga cgctgctgct tctgctactg
        61 ttgctgccgc ctgcggtgct gtcgcccggg gcggcggcgc agccactgcc cgactgctgc
        121 cgtcaaaaga cgtgctcctg ccgcctctac gagctgctac acggcgctgg caatcacgcg
        181 gccggcatcc tcactctggg caagcggcgg ccagggcccc cgggcctgca aggccggctg
        241 cagcgcctcc tgcaggccag cggcaaccac gcagcgggca tcctgaccat gggccgccgc
        301 gcaggcgcag agccagcgcc gcgcctctgc ccggggcgcc ggtgtctcgc tgcggctgcc
        361 tcatctgtag cgccgggagg acggtctggg atctgagccc tagcccggcc ctctcccctc
        421 tgcccgcccg aggtcagccc cccagaaaaa g

        本人查阅资料有人做过用他的引物却怎么也扩增不出来,扩增出来的不是我的目的片段大小,我的目的片段大小为356,可扩增出来的主要为二百多的片段。正义引物5‘-CGCTGCTGCTTCTGCTACTG-3’反义引物5‘-TCAGATCCCAGACCGTCCTC-3’
        请各位高手帮帮,我急用。
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