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        用DAPI进行细胞染色

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        用DAPI进行细胞染色

        DAPI的配置

        Stock solution of 1mg/ml in ddH2O; working solution of 0.25mg/ml to 50mg/ml in ddH2O or PGM.

        1.Place sample on slide.

        2.Add a few drops working solution.

        3.Squash and view.

        Staining � DAPI Staining for Cells:

        Separately DAPI staining:

        • Wash the cells twice with PBS

        • Incubate with 1: 750 of stock DAPI for 5 min in dark

        • Wash 2 x 5 min with PBS in dark

        • Rinse with water for a few seconds

        • Air dry at RT in dark

        • Mount with antifade solution (Vectashield, Co#: H-1000, Vector Lab)

        5 µl for each d12 mm coverslip, 25 µl for square coverslip and 50µl for full size coverslip

        • Seal the coverslip with nail polish

        • Store in 4 C up to 2 weeks

        DAPI stock

        • 4’, 6-Diamidino-2-phenylindole dihydrochloride hydrate (DAPI) from Sigma (Cat# D-9542), 1 mg 1mg/0.5 ml in H2O and stocked at �20 in the dark

        Staining with antifade mounting solution:

        • Wash the cells twice with PBS

        • Mount with antifade solution with DAPI (Vectashield, Co#: H-1200,Vector Lab, 1.5 µg/ml) at 1:3 dilution with Vectashield H-1000 Volume used as above

        • Store in 4 C up to 2 weeks

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