Caspase 3/7和DAPI凋亡双染试剂盒

Caspase 3/7和DAPI凋亡双染试剂盒
(Caspase 3/7 and DAPI Apoptosis Double Staining Kit)

货号：KTA3028 | 产品中心：细胞分析 | 产品类型：细胞凋亡/周期

产品概述

该试剂盒专为B2B研究应用设计，适用于基于细胞样本的凋亡机制分析。通过双染法同时检测Caspase 3/7酶活性与细胞状态，支持荧光信号观察（荧光激发/发射波长详见背景描述），适用于细胞分析领域中的凋亡过程研究。

核心工作原理

Caspase 3/7 Green作为底物可被激活的Caspase 3/7酶切割，释放高亲和力DNA染料结合后产生绿色荧光（Ex/Em: 488 nm/525 nm），特异性指示凋亡早期细胞。DAPI则结合双链DNA，在细胞膜完整性丧失时（如凋亡晚期或坏死）发出蓝色荧光（Ex/Em: 358 nm/461 nm）。双重信号可实现凋亡与坏死细胞的有效区分。

关键组分

• Caspase 3/7 Green：细胞膜通透性底物，基于DEVD识别序列。
• DAPI（4',6-二脒基-2-苯基吲哚二`盐`酸`盐）：高亲和力DNA染料。

规格与订购选项

样本类型：细胞 | 应用领域：细胞状态分析（如凋亡起始监测）

保存与运输指南

建议收到货后避光保存于-20°C，有效期为12个月。运输采用冰袋（蓝冰）条件，确保试剂稳定性。

重要注意事项

警告：本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。

推荐搜索别名：Caspase 3/7、DAPI。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Caspase 3/7 and DAPI Apoptosis Double Staining Kit
(Caspase 3/7 and DAPI Apoptosis Double Staining Kit)

Catalog Number: KTA3028 | Product Center: Cell Analysis | Product Type: Apoptosis/Cell Cycle

Product Overview

This kit is specifically designed for B2B research applications and is suitable for apoptosis mechanism analysis based on cell samples. Through double staining, it simultaneously detects Caspase 3/7 enzyme activity and cell status, supporting fluorescence signal observation (fluorescence excitation/emission wavelengths are detailed in the background description). It is applicable for studying apoptosis processes in the field of cell analysis.

Core Working Principle

Caspase 3/7 Green, as a substrate, can be cleaved by activated Caspase 3/7 enzymes, releasing a high-affinity DNA dye that produces green fluorescence (Ex/Em: 488 nm/525 nm) upon binding, specifically indicating early apoptotic cells. DAPI binds to double-stranded DNA, emitting blue fluorescence (Ex/Em: 358 nm/461 nm) when cell membrane integrity is lost (e.g., in late apoptosis or necrosis). Dual signals enable effective differentiation between apoptotic and necrotic cells.

Key Components

• Caspase 3/7 Green: Cell membrane-permeable substrate based on the DEVD recognition sequence.
• DAPI (4',6-Diamidino-2-phenylindole dihydrochloride): High-affinity DNA dye.

Specifications and Ordering Options

Sample Type: Cells | Application Field: Cell status analysis (e.g., apoptosis initiation monitoring)

Storage and Shipping Guidelines

Upon receipt, it is recommended to store the product protected from light at -20°C, with a shelf life of 12 months. Shipping is conducted under ice pack (blue ice) conditions to ensure reagent stability.

Important Notes

Warning: The products listed herein are for research use only and are not intended for human or clinical diagnostic use. The applications recommended for our products do not imply that we suggest using our products to violate any patents or licenses. We assume no responsibility for any patent infringement or other violations that may arise from the use of this product.

Recommended search aliases: Caspase 3/7, DAPI.

Note: The above information may be subject to changes due to product optimization and upgrades. For specific details, please consult Yakeyin Company.