SARS-CoV-2 Spike RBD (T478K) Protein (His Tag)

SARS-CoV-2 Spike RBD (T478K) Protein (His Tag)（产品说明）
蛋白名称：SARS-CoV-2 Spike RBD (T478K) Protein (His Tag)
蛋白构建：A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutation T478K) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus. The mutation were identified in the SARS-CoV-2 variant (known as variant B.1.617.2) which emerged in the India.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE.
蛋白活性：Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (T478K) Protein (His Tag)(Cat:40592-V08H91), the EC50 of SARS-CoV-2 Spike RBD (T478K) Protein (His Tag)(Cat: 40592-V08H91) is 6-48 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Ser
蛋白分子量：The recombinant SARS-CoV-2 Spike RBD consists of 235 amino acids and predicts a molecular mass of 26.7 kDa. As a result of glycosylation, it migrates as an approximately 34.9 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Arg319-Phe541
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.