- ￥4500
- 义翘神州
- 40592-V08H90
- 北京
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
SARS-CoV-2 Spike RBD (L452R,T478K) Protein (His Tag)
- 保质期：
12个月
- 供应商：
北京义翘神州科技股份有限公司
- 保存条件：
-20℃ to -80℃
- 规格：
100 µg
SARS-CoV-2 Spike RBD (L452R,T478K) Protein (His Tag): 产品信息
纯度> 95 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Testing in progress
蛋白构建A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutations L452R,T478K) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.617.2) which emerged in the India.
表达宿主HEK293 Cells
种属SARS-CoV-2
预测 N 端Arg 319
分子量The recombinant SARS-CoV-2 Spike RBD consists of 234 amino acids and predicts a molecular mass of 26.6 kDa. As a result of glycosylation, it migrates as an approximately 36.9 kDa band in SDS-PAGE under reducing conditions.
缓冲液Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
纯度> 95 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Testing in progress
蛋白构建A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutations L452R,T478K) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.617.2) which emerged in the India.
表达宿主HEK293 Cells
种属SARS-CoV-2
预测 N 端Arg 319
分子量The recombinant SARS-CoV-2 Spike RBD consists of 234 amino acids and predicts a molecular mass of 26.6 kDa. As a result of glycosylation, it migrates as an approximately 36.9 kDa band in SDS-PAGE under reducing conditions.
缓冲液Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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文献和实验该产品被引用文献
1, Li, C; et al. Broad neutralization of SARS-CoV-2 variants by an inhalable bispecific single-domain antibody. Cell, PMID: 35344711
2, Emmenegger, M; et al. Both COVID-19 infection and vaccination induce high-affinity cross-clade responses to SARS-CoV-2 variants. iScience, PMID: 35875683
3, Zhang, J; et al. Mechanosensing view of SARS-CoV-2 infection by a DNA nano-assembly. Cell Reports Physical Science, PMID: 36157982
4, Kim, JW; et al. Development and Characterization of Phage-Display-Derived Novel Human Monoclonal Antibodies against the Receptor Binding Domain of SARS-CoV-2. Biomedicines, PMID: 36552031
5, Wang, Y; et al. Biparatopic antibody BA7208/7125 effectively neutralizes SARS-CoV-2 variants including Omicron BA.1-BA.5. Cell discovery, PMID: 36609558
6, Emmenegger, M; et al. Protocol to determine antibody affinity and concentration in complex solutions using microfluidic antibody affinity profiling. STAR protocols, PMID: 36853663
7, Wang, M; et al. Oxalic acid blocked the binding of spike protein from SARS-CoV-2 Delta (B.1.617.2) and Omicron (B.1.1.529) variants to human angiotensin-converting enzymes 2. PloS one, PMID: 37200310
8, Yu, X; et al. Discovery of Peptidic Ligands against the SARS-CoV-2 Spike Protein and Their Use in the Development of a Highly Sensitive Personal Use Colorimetric COVID-19 Biosensor. ACS sensors, PMID: 37253267
9, Zhang, RG; et al. SARS-CoV-2 spike protein receptor binding domain promotes IL-6 and IL-8 release via ATP/P2Y2 and ERK1/2 signaling pathways in human bronchial epithelia. Molecular immunology, PMID: 38359646
10, Chen, Y; et al. Structure-Based Optimization of One Neutralizing Antibody against SARS-CoV-2 Variants Bearing the L452R Mutation. Viruses, PMID: 38675908
11, Park, S; et al. An ancestral SARS-CoV-2 vaccine induces anti-Omicron variants antibodies by hypermutation. Nature communications, PMID: 38643233 1
2, Ana-Sosa-Batiz, F; et al. A humanised ACE
2, TMPRSS
2, and FCGRT mouse model reveals the protective efficacy of anti-receptor binding domain antibodies elicited by SARS-CoV-2 hybrid immunity. EBioMedicine, PMID: 40020261 1
3, Thompson, A; et al. Identification of an Unnatural Sulfated Monosaccharide as a High-Affinity Ligand for Pan-Variant Targeting of SARS-CoV-2 Spike Glycoprotein. ACS chemical biology, PMID: 40358361
2, Emmenegger, M; et al. Both COVID-19 infection and vaccination induce high-affinity cross-clade responses to SARS-CoV-2 variants. iScience, PMID: 35875683
3, Zhang, J; et al. Mechanosensing view of SARS-CoV-2 infection by a DNA nano-assembly. Cell Reports Physical Science, PMID: 36157982
4, Kim, JW; et al. Development and Characterization of Phage-Display-Derived Novel Human Monoclonal Antibodies against the Receptor Binding Domain of SARS-CoV-2. Biomedicines, PMID: 36552031
5, Wang, Y; et al. Biparatopic antibody BA7208/7125 effectively neutralizes SARS-CoV-2 variants including Omicron BA.1-BA.5. Cell discovery, PMID: 36609558
6, Emmenegger, M; et al. Protocol to determine antibody affinity and concentration in complex solutions using microfluidic antibody affinity profiling. STAR protocols, PMID: 36853663
7, Wang, M; et al. Oxalic acid blocked the binding of spike protein from SARS-CoV-2 Delta (B.1.617.2) and Omicron (B.1.1.529) variants to human angiotensin-converting enzymes 2. PloS one, PMID: 37200310
8, Yu, X; et al. Discovery of Peptidic Ligands against the SARS-CoV-2 Spike Protein and Their Use in the Development of a Highly Sensitive Personal Use Colorimetric COVID-19 Biosensor. ACS sensors, PMID: 37253267
9, Zhang, RG; et al. SARS-CoV-2 spike protein receptor binding domain promotes IL-6 and IL-8 release via ATP/P2Y2 and ERK1/2 signaling pathways in human bronchial epithelia. Molecular immunology, PMID: 38359646
10, Chen, Y; et al. Structure-Based Optimization of One Neutralizing Antibody against SARS-CoV-2 Variants Bearing the L452R Mutation. Viruses, PMID: 38675908
11, Park, S; et al. An ancestral SARS-CoV-2 vaccine induces anti-Omicron variants antibodies by hypermutation. Nature communications, PMID: 38643233 1
2, Ana-Sosa-Batiz, F; et al. A humanised ACE
2, TMPRSS
2, and FCGRT mouse model reveals the protective efficacy of anti-receptor binding domain antibodies elicited by SARS-CoV-2 hybrid immunity. EBioMedicine, PMID: 40020261 1
3, Thompson, A; et al. Identification of an Unnatural Sulfated Monosaccharide as a High-Affinity Ligand for Pan-Variant Targeting of SARS-CoV-2 Spike Glycoprotein. ACS chemical biology, PMID: 40358361
相关实验
HIS 标签蛋白纯化效果不理想，宝宝心里苦呀。今天，小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的 HIS 标签蛋白。该问题主要可以分为以下两个方面： 1、HIS 标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高，容易导致蛋白炭化；功率过低，蛋白释放不出来。 建议 改变超声功率，同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素，如：金属离子螫合剂、强还原剂、过高的咪唑浓度。同时，优化缓冲
蛋白标签（protein tag）是指利用DNA体外重组技术，与目的蛋白一起融合表达的一种多肽或者蛋白，以便于目的蛋白的表达、检测、示踪和纯化等。随着技术的不断发展，研究人员相继开发出了具有各种不同功能的蛋白标签。 一、 新型蛋白标签 蛋白标签应用极为广泛，但仍有两个问题不容忽视：（1）蛋白标签以DNA形式编码，它需要转录并翻译成蛋白后才能发挥作用，而这种作用方式本身就是一种缺陷。例如，常用的荧光蛋白标签，在显微镜下观察时，其显像不如人工合成的荧光基团好，但是这种人工合成的荧
[资源][原创] 蛋白标签纯化技术——CBP（钙调蛋白结合肽）
基于ＣＢＰ标签技术的蛋白质表达与纯化系统 （用于高水平蛋白质表达和单步骤纯化）Protein Expression and Purification System是一种简单、温和、有效的方法，包括一个含有 calmodulin-binding peptide tag 钙调蛋白结合肽标签的pCAL 表达载体和一个用于分离的钙激酶亲合树脂，可快速敏感的捕捉标签融合蛋白。T7 RNA polymerase-based pCAL 载体能够快速克隆钙调蛋白结合肽标签融合蛋白。CBP标记的蛋白在低浓度钙
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重组SARS-CoV-2 Spike RBD (L452R,T478K) 蛋白 (His 标签)
￥4500
