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- ab235672
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- 2025年07月30日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
GFP Quantitation Kit
- 保存条件：
ab235672
- 供应商：
上海研卉生物科技有限公司
- 库存：
大量
- 规格：
100次分析
GFP绿色荧光蛋白定量试剂盒
主要功能和细节
化验类型：定量
检测方法：荧光
平台：微孔板阅读器
样品类型：细胞培养提取物，细胞裂解物，组织提取物，组织匀浆，组织裂解物
Kit Summary:
• Detection method- Fluorescence (Ex/Em 488/507 nm)
• Sample type- Cell and Tissue culture
• Species reactivity- Mammalian
• Application- It detects a wide range of GFP concentration (0.01-10 ug/ml)
Features & Benefits:
• Simple procedure; takes ~ 20 minutes
• Fast and convenient
Kit components:
• GFP Assay Buffer
• GFP Standard (1 µg/µl)
• GFP Quench Solution
Description:
Green Fluorescent Protein (GFP) is a spontaneously fluorescent protein originally isolated from the jellyfish Aequorea Victoria (Chalfie et al, 1994). GFP has also been recombinant modified and enhanced (called EGFP). GFP and EGFP have been widely used as a valuable reporter molecule for in vivo visualization of gene expression events in a variety of cell types and organisms. Since GFP requires no additional substrates or cofactors, GFP fluorescence can be easily detected under fluorescence microscope. However, most imaging studies of GFP are only qualitative. Since quantitative analysis of GFP expression level in cells or tissues are more informative and have wide applications, BioVision has developed the GFP Quantification Kit which quantifies GFP in 96 micro-plate format. Cells or tissues can be homogenized directly in the GFP Assay Buffer. The quantity of GFP is determined by comparing its fluorescence with that of GFP standard. The kit can detect a wide range of GFP concentration (0.01-10 µg/ml). A GFP quench solution is also provided for determining auto-fluorescence of cell or tissue extracts. Each kit provides sufficient reagents to perform up to 100 assays, Ex/Em = 488/507 nm.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.
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文献和实验ab235672 被引用在 4 文献中.
- Carrillo Sanchez B et al. GFP-tagging of extracellular vesicles for rapid process development. Biotechnol J 17:e2100583 (2022). PubMed: 35332662
- Poudel S et al. Oral Delivery of Nucleic Acids with Passive and Active Targeting to the Intestinal Tissue Using Polymer-Based Nanocarriers. Pharmaceutics 13:N/A (2021). PubMed: 34371766
- Dos Santos NV et al. Revealing a new fluorescence peak of the enhanced green fluorescent protein using three-dimensional fluorescence spectroscopy. RSC Adv 9:22853-22858 (2019). PubMed: 35514499
- Wang Y et al. Soybean miR172c targets the repressive AP2 transcription factor NNC1 to activate ENOD40 expression and regulate nodule initiation. Plant Cell 26:4782-801 (2014). PubMed: 25549672
dmdfe 本人将GFP基因后面加了一段大约110bp的序列，插入PET28A的BamH1和Hind3位点，最后在BL21中表达。 经SDS-PAGE验证表明： 目的蛋白 大量存在菌体裂解后沉淀即包涵体中，但是该沉淀无色。 少量存在于上清中，上清显微弱的绿色。 看文献大量报道GFP融合蛋白几乎都形成可溶性表达，目的蛋白在上清中，并且诱导条件没有太大要求。 想请教一下各位做过GFP原核表达的经验
绿色荧光蛋白GFP来源及生化特性: GFP 主要来自两种海洋无脊椎动物,西南太平洋水母———Aequorea victoria 和佐治亚沿海水域的三色堇———Renilla renifomis 。上述来源的GFP ,其原始发光蛋白基团具有将蓝色的化学发光演变为绿色荧光的生物发光特性。Plinythe Elder 于公元1 世纪最早报道了这种生物发光现象。20 世纪60 年代以来,Blinks 等实验室先后研究了GFP 的生物化学特性。野生型GFP (wt2GFP) 的分子量约为27kD
步是用特异性的抗体检测出已经印迹在膜上的所要研究的相应抗原。免疫检测的方法可以是直接的和间接的。现在多用间接免疫酶标的方法，在用特异性的第一抗体染色后，再用酶标的第二抗体（碱性磷酸酶（Ap）或辣根过氧化物酶（HRP）标记的抗第一抗体的抗体）染色，再加酶的底物显色，通过膜上的颜色或X光底片上暴光的条带来显示抗原的存在。 为了使初学者能够在实验过程中观察到所要检测的目的蛋白，本实验使用的是非变性的聚丙烯酰胺凝胶电泳，以保持GFP的天然活性，利用荧光对其进行追踪。此外，实验中用国产的HRP
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