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5mL×2
蛋白上样缓冲液(变性,非还原型,5×)
Protein Sample Loading Buffer(Denaturing, non-Reducing, 5×)
本产品冰袋运输;保存于-20℃,保质期12个月。
货号规格
goodLT103 1 mL×10
产品简介
good本品是一种经过改良的以溴酚蓝为染料,5倍浓缩的蛋白上样缓冲液。本产品中含有变性试剂,但是不含DTT、巯基乙醇等还原剂。可以用于常规的变性非还原型聚bin烯酰胺凝胶电泳的蛋白样品上样。
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相关论文
good1. Chen C, Liu W, Guo J, et al. Nuclear m6A reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos[J]. Protein & cell, 2021, 12(6): 455-474.(IF 14.870)
good2. Huang R, Zhang L, Jin J, et al. Bruceine D inhibits HIF-1α-mediated glucose metabolism in hepatocellular carcinoma by blocking ICAT/β-catenin interaction[J]. Acta Pharmaceutica Sinica B, 2021, 11(11): 3481-3492.(IF 11.413)
good3. Wei, Y., Tian, C., Zhao, Y., Liu, X., Liu, F., Li, S., ... & Zhou, T. (2020). MRG15 orchestrates rhythmic epigenomic remodelling and controls hepatic lipid metabolism. Nature Metabolism, 2(5), 447-460.(IF 13.511)
good4. YU, Chen, et al. Study of chondroitin sulfate E oligosaccharide as a promising complement C5 inhibitor for osteoarthritis alleviation. Materials Science and Engineering: C, 2021, 127: 112234.(IF 7.328)
good5. LI, Tao, et al. Differential expression profiles of long noncoding RNAs and mRNAs in human bone marrow mesenchymal stem cells after exposure to a high dosage of dexamethasone. Stem Cell Research & Therapy, 2021, 12.1: 1-19.(IF 6.832)
good6. Yang M, Qiu R, Wang W, et al. P2X7 Receptor Antagonist Attenuates Retinal Inflammation and Neovascularization Induced by Oxidized Low-Density Lipoprotein[J]. Oxidative medicine and cellular longevity, 2021, 2021.(IF 6.543)
good7. WANG, Xiao-Qing, et al. PTEN in prefrontal cortex is essential in regulating depression-like behaviors in mice. Translational psychiatry, 2021, 11.1: 1-12.(IF 6.222)
good8. Huang, J., Tong, X., Zhang, Y., Zhang, L., Wang, L., Dongguang, W., ... & Fan, H. (2020). Hyperoside attenuates bleomycin-induced pulmonary fibrosis development in mice. Frontiers in Pharmacology, 11, 1430. (IF 5.810)
good9. ZHUANG, Qian, et al. Astragalus Polysaccharides Ameliorate Diet-Induced Gallstone Formation by Modulating Synthesis of Bile Acids and the Gut Microbiota. Frontiers in Pharmacology, 2021, 12.(IF 5.810)
good10. WANG, Jian‐tao, et al. A novel palmitic acid hydroxy stearic acid (5‐PAHSA) plays a neuroprotective role by inhibiting phosphorylation of the m‐TOR‐ULK1 pathway and regulating autophagy. CNS neuroscience & therapeutics, 2021, 27.4: 484-496.(IF 5.243)
good11. Gao Z, Gao X, Fan W, et al. Bisphenol A and genistein have opposite effects on adult chicken ovary by acting on ERα/Nrf2-Keap1-signaling pathway[J]. Chemico-Biological Interactions, 2021, 347: 109616.(IF 5.192)
good12. FANG, Xiaolong, et al. Modulation of evening complex activity enables north-to-south adaptation of soybean. Science China Life Sciences, 2021, 64.2: 179-195.(IF 5.037)
good13. Xu Y, Jiang Y, Jia B, et al. Icariin stimulates osteogenesis and suppresses adipogenesis of human bone mesenchymal stem cells via miR-23a-mediated activation of the Wnt/β-catenin signaling pathway[J]. Phytomedicine, 2021, 85: 153485.(IF 5.034)
good14. Zhou Q, He D X, Deng Y L, et al. MiR-124-3p targeting PDE4B attenuates LPS-induced ALI through the TLR4/NF-κB signaling pathway[J]. International Immunopharmacology, 2022, 105: 108540.(IF 4.932)
good15. Chen J, Yan J, Li S, et al. Atorvastatin inhibited TNF-α induced matrix degradation in rat nucleus pulposus cells by suppressing NLRP3 inflammasome activity and inducing autophagy through NF-κB signaling[J]. Cell Cycle, 2021, 20(20): 2160-2173.(IF 4.534)
good16. He Z, Liu X X, Liu X, et al. The role of MEG3 in the proliferation of palatal mesenchymal cells is related to the TGFβ/Smad pathway in TCDD inducing cleft palate[J]. Toxicology and Applied Pharmacology, 2021, 419: 115517.(IF 4.219)
good17. Xu, Y., Jiang, Y., Wang, Y., Zhao, Z., & Li, T. (2020). LINC00473 rescues human bone marrow mesenchymal stem cells from apoptosis induced by dexamethasone through the PEBP1‑mediated Akt/Bad/Bcl‑2 signaling pathway. International Journal of Molecular Medicine. (IF 4.101)
good18. ZHANG, Lujia, et al. Autosomal Recessive Retinitis Pigmentosa Associated with Three Novel REEP6 Variants in Chinese Population. Genes, 2021, 12.4: 537.(IF 4.096)
good19. Qiu, R., Yang, M., Wang, W., Liu, J., Yang, L., & Lei, B. (2020). The Protective Effects of VVN001 on LPS-Induced Inflammatory Responses in Human RPE Cells and in a Mouse Model of EIU. Inflammation, 1-15. (IF 4.092)
good20. Li S, Huang Z, Zhu Y, et al. Bromodomain-containing protein 7 regulates matrix metabolism and apoptosis in human nucleus pulposus cells through the BRD7-PI3K-YAP1 signaling axis[J]. Experimental Cell Research, 2021, 405(2): 112658.(IF 3.905)
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文献和实验SELDI技术闲谈 我总是在黑白之间徘徊,在那灰色的地带游走。 一,Seldi的原理。 Seldi技术,说来不复杂,ProteinChip和TOF-MS两个部分,有的专家说“蛋白质芯片”这个叫法不正确,当然了,我们以前讲的某某“芯片”是微点阵“Microarray”,不是“Chip”,翻译的问题而已。蛋白质芯片是将传统的生物色谱的表面集团固定到金属片基上,比如Q、NTA、C18这些,所以蛋白质芯片也分成了离子交换、金属螯合、正相反相、疏水、亲和,基本上与常用的色谱方法一一
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