<毕赤酵母>Overview of Protein Expression in Pichia pastoris-<Copyright ©2004 by John W
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UNIT 5.7 Overview of Protein Expression in Pichia pastoris
David R. Higgins1
1 Invitrogen Corporation, San Diego, California
John E. Coligan, Ben M. Dunn, David W. Speicher, and Paul T. Wingfield (eds.)
Current Protocols in Protein Science
Copyright © 2003 John Wiley & Sons, Inc. All rights reserved.
DOI: 10.1002/0471140864.ps0507s02
Online Posting Date: May, 2001
Print Publication Date: December, 1995
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Abstract
Pichia pastoris is a methylotrophic yeast and can be used as a heterologous expression system. This microorganism is as easy to manipulate as Escherichia coli, but has many of the advantages of eukaryotic expression (e.g., protein processing, folding, and post-translational modifications), and it is faster, easier, and cheaper to use than other eukaryotic expression systems, such as baculovirus or mammalian tissue culture. It also generally yields higher expression levels. This overview discusses important considerations for the use of Pichia pastoris, including strains for expression, expression plasmids, transformation by integration, and post-translational modifications. Examples of expression are given and finally, legal issues regarding patent rights for heterologous protein expression in Pichia pastoris are described.
David R. Higgins1
1 Invitrogen Corporation, San Diego, California
John E. Coligan, Ben M. Dunn, David W. Speicher, and Paul T. Wingfield (eds.)
Current Protocols in Protein Science
Copyright © 2003 John Wiley & Sons, Inc. All rights reserved.
DOI: 10.1002/0471140864.ps0507s02
Online Posting Date: May, 2001
Print Publication Date: December, 1995
--------------------------------------------------------------------------------
Abstract
Pichia pastoris is a methylotrophic yeast and can be used as a heterologous expression system. This microorganism is as easy to manipulate as Escherichia coli, but has many of the advantages of eukaryotic expression (e.g., protein processing, folding, and post-translational modifications), and it is faster, easier, and cheaper to use than other eukaryotic expression systems, such as baculovirus or mammalian tissue culture. It also generally yields higher expression levels. This overview discusses important considerations for the use of Pichia pastoris, including strains for expression, expression plasmids, transformation by integration, and post-translational modifications. Examples of expression are given and finally, legal issues regarding patent rights for heterologous protein expression in Pichia pastoris are described.
