pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation E-P=ELISA (Peptide)
Reactivity Key: H=Human R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications |
Reactivity |
Sensitivity |
MW (kDa) |
Source |
| W IP E-P |
H R Mk |
Endogenous |
110 |
Rabbit |
| Protocols |
-
9307:
- ELISA Peptide , Immunoprecipitation , Western Blotting
|
| Specificity / Sensitivity |
Phospho-Rb (Ser780) Antibody detects endogenous levels of Rb only when phosphorylated at Ser780. The antibody does not cross-react with Rb phosphorylated at other sites. |
| Source / Purification |
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser780 of human Rb. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting 
Western blot analysis of extracts from human fibroblasts synchronized by serum deprivation, using Phospho-Rb (Ser780) Antibody. Cells were synchronized for 24 hours then released by addition of serum and harvested at the times indicated. Cell cycle progression was verified by cyclin analysis and FACS. (Provided by John Boylan, Dupont/Merck, Delaware.)
|
| Background |
The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).
- Sherr, C.J. (1996) Science 274, 1672-7.
- Nevins, J.R. (1992) Science 258, 424-9.
- Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90.
- Hu, Q.J. et al. (1990) EMBO J 9, 1147-55.
- Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83.
- Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.
- Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301.
- Kitagawa, M. et al. (1996) EMBO J 15, 7060-9.
- Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.
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| Application References |
- Chiariello, M. et al. (2000) Regulation of cyclin-dependent kinase (Cdk) 2 Thr-160 phosphorylation and activity by mitogen-activated protein kinase in late G1 phase. Biochem. J. 349, 869-876. Applications: Western Blotting
- Fujimoto, K. et al. (2000) Inhibition of pRb phosphorylation and cell cycle progression by an antennapedia-p16(INK4A) fusion peptide in pancreatic cancer cells. Cancer Lett. 159, 151-158. Applications: Western Blotting
- Huang, H. et al. (2004) Androgens repress Bcl-2 expression via activation of the retinoblastoma (RB) protein in prostate cancer cells. Oncogene 23, 2161-2176. Applications: Western Blotting
- Feng, L. X. et al. (2000) Stem cell factor/c-kit up-regulates cyclin D3 and promotes cell cycle progression via the phosphoinositide 3-kinase/p70S6 kinase pathway in Spermatogonia. J. Biol. Chem. 275, 25572-25576. Applications: Western Blotting
- Dupont, J. et al. (2000) The potentiation of estrogen on insulin-like growth factor I action in MCF-7 human breast cancer cells includes cell cycle components. J. Biol. Chem. 275, 35893-35901. Applications: Western Blotting
- Faenza, I. et al. (2000) A role for nuclear phospholipase c beta 1 in cell cycle control. J. Biol. Chem. 275, 30520-30524. Applications: Western Blotting
- Falco, A. et al. (2012) Oncogene , . Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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