- 询价
- Cell Signaling Technology
- 9407
- USA
- 2025年10月17日
- W, IF-IC, F
- Rabbit
- H,M,R,Mk
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- 详细信息
- 技术资料
- 抗体英文名：
DDX6/RCK Antibody
- 抗原：
synthetic peptide corresponding to residues near the carboxy terminus of human DDX6/RCK protein
- 应用范围：
W, IF-IC, F
- 宿主：
Rabbit
- 保质期：
详见说明书
- 供应商：
CST
- 库存：
大量
- 级别：
详见MSDS文件
- 适应物种：
H,M,R,Mk
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC F | H M R Mk | Endogenous | 54 | Rabbit |
| Protocols |
* Product-specific protocol. |
|---|---|
| Specificity / Sensitivity | DDX6/RCK Antibody detects endogenous levels of total DDX6/RCK protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DDX6/RCK protein. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from various cell types using DDX6/RCK Antibody. Flow Cytometry
Flow cytometric analysis of HeLa cells using DDX6/RCK Antibody (blue) compared to a nonspecific negative control antibody (red). IF-IC
Confocal immunofluorescent analysis of UV-treated C2C12 cells using DDX6/RCK Antibody (green) and α-Tubulin (DM1A) Mouse mAb #3873 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | DDX6, also known as RCK and p54, was identified as a proto-oncogene product and is a member of the ATP-dependent DEAD box helicase family (1,2). This protein interacts with translation initiation factor eIF4E in the cytoplasmic P-bodies (3) and represses mRNA translation (4). DDX6 is a component of the miRNA induced silencing complex (miRISC) and interacts with Argonaute 1 (Ago1) and Argonaute 2 (Ago2) proteins in vitro and in vivo (5), functioning in miRNA-mediated translational repression (5). Depletion of DDX6 leads to the disruption of cytoplasmic P-bodies indicating that it is required for P-body formation (5). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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