Phospho-IκBα (Ser32/36) (5A5)

9246:

IHC / Paraffin , Immunoprecipitation , Western Blotting

Phospho-IκBα (Ser32/36) (5A5) Mouse mAb detects endogenous levels of IκBα only when phosphorylated at Ser32/36.

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser32/36 of human IκBα.

Western Blotting

Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24 h) and treated with 1 μg/ml LPS for the indicated times, using Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (upper) and IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814 (lower).

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated orTNF-α-treated (#2169, 20 ng/ml) for 5 minutes, using Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (upper) or IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814 (lower).

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast tumor using Phospho-IκBα (Ser32/36) (5A5) Mouse mAb.

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
4. Brown, K. et al. (1995) Science 267, 1485-8.
5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
8. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.

• Howe, C.J. et al. (2002) J Biol Chem 277, 30469-76. Applications: Western Blotting
• Casciati, A. et al. (2002) J Neurochem 83, 1019-29. Applications: Western Blotting
• Gao, H. et al. (2004) Mol Cell 14, 303-17. Applications: Western Blotting
• Nair, A. et al. (2003) Oncogene 22, 50-8. Applications: IHC-P (paraffin)
• Schultz, C. et al. (2006) Mol Cell Neurosci 33, 68-80. Applications: Western Blotting
• Basak, S. et al. (2007) Cell 128, 369-81. Applications: Western Blotting
• Chen, Y. et al. (2007) J Immunol 178, 49-57. Applications: Western Blotting
• Wright, A. et al. (2007) Dev Cell 13, 705-16. Applications: IHC-P (paraffin)
• Lou, H. and Kaplowitz, N. (2007) J Biol Chem 282, 29470-81. Applications: Kinase Assay Western Blotting
• Saitoh, Y. et al. (2008) Blood 111, 5118-29. Applications: Western Blotting
• Luedde, T. et al. (2008) Proc Natl Acad Sci U S A 105, 9733-8. Applications: Western Blotting
• Vince, J.E. et al. (2008) J Cell Biol 182, 171-84. Applications: Western Blotting
• Mabilleau, G. and Sabokbar, A. (2009) PLoS ONE 4, e4173. Applications: Western Blotting
• Tokunaga, F. et al. (2009) Nat Cell Biol 11, 123-32. Applications: Western Blotting
• Chiron, D. et al. (2009) J Immunol 182, 4471-8. Applications: Western Blotting
• Turner, N.A. et al. (2009) Am J Physiol Heart Circ Physiol 297, H1117-27. Applications: Western Blotting
• Kwong, C. et al. (2011) J Immunol 186, 1781-9. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

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• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 8125 SignalStain^® Boost IHC Detection Reagent (HRP, Mouse)

For Research Use Only. Not For Use In Diagnostic Procedures.