- 询价
- Cell Signaling Technology
- 4088
- USA
- 2025年09月10日
- IP
- H,M,R,Mk,Dg,B,Pg
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (Sepharose Bead Conjugate)
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser32/36 of human IκBα
- 应用范围：
IP
- 适应物种：
H,M,R,Mk,Dg,B,Pg
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 供应商：
CST
- 库存：
大量
- 标记物：
Sepharose Bead
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
400 ul (40 immunoprecipitations)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 400 ul (40 immunoprecipitations) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey B=Bovine Dg=Dog Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| IP | H M R Mk Dg (B) (Pg) | Endogenous | 40 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (Sepharose Bead Conjugate) detects endogenous levels of IκBα only when phosphorylated at Ser32/36. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser32/36 of human IκBα. IP
Immunoprecipitation of NIH/3T3 cell lysates, untreated or TNF-α-treated, using Mouse IgG (Sepharose Bead Conjugate) #3420 (Lanes 1 and 2) and Immobilized Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (Bead Conjugate) (Lanes 3 and 4). The blot was probed using Phospho-IκBα (Ser32/36) (5A5) Mouse mAb #9246. |
| Description | This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of phosphorylated IκBα. |
| Background | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
david9931 请问各位大侠，有没有订做过磷酸化的丝氨酸或磷酸化的苏氨酸抗体，哪家公司做的比较好，小弟想订做目的蛋白特异位点的丝氨酸、苏氨酸的磷酸化的抗体，帮忙给指点下，多谢啦 euthor_hsu 这种抗体定做很难。毕竟要的是抗一个特异的氨基酸的磷酸化位点。 就我所知cisbio有anti-phosphothronine 和anti-phosphotyrosine的抗体，PAb和MAb都有，但是没有anti
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
4.5 (without removing prehybridization solution.) C. Repeat the addition of the 2XSSC wash twice more. Remove the mix and wash twice, 30 min each time, in 2XSSC pH7/0.1% CHAPS 70 °C. Wash twice, 10 min each, in Maleic Acid Buffer ( MAB; 100 mM maleic acid
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