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- 详细信息
- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
- 免疫类型：
IgG1
- 细胞形态：
淋巴样
- ATCC Number：
CRL-8017™
- 生长状态：
悬浮生长
- 运输方式：
冻存运输
| Designations: | H25B10 | ||
| Depositors: | Massachusetts General Hospital | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against hepatitis B virus surface antigen (HBsAg) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with hepatitis B virus surface antigen. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 85%; fetal bovine serum, 15% |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| References: | 3495: Wands JR, Zurawski VR Jr.. Process for producing antibodies to hepatitis virus and cell lines therefor. US Patent 4,271,145 dated Jun 2 1981 28236: Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997. PubMed: 8985336 |
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H25B10
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