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- 生长状态：
贴壁生长
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 年限：
adult
- 细胞形态：
上皮样
- 相关疾病：
腺癌
- 器官来源：
胰腺
- 运输方式：
冻存运输
- ATCC Number：
CRL-2547™
| Designations: | Panc 10.05 | ||
| Depositors: | EM Jaffee | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens deposited as human | ||
| Morphology: | epithelial |
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| Source: | Organ: pancreas Disease: adenocarcinoma |
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| Cellular Products: | cytokeratins 7 and 18 [50655 ] | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1992 | ||
| Tumorigenic: | Yes | ||
| Oncogene: | K-ras + | ||
| Antigen Expression: | MHC class I +; MHC class II - | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9,12 D5S818: 13 D7S820: 8,9 THO1: 6,9.3 TPOX: 11 vWA: 16 |
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| Age: | adult | ||
| Gender: | male | ||
| Ethnicity: | White | ||
| Comments: | Panc 10.05 is a pancreatic adenocarcinoma epithelial cell line derived in 1992 from a primary tumor removed from the head-of-the-pancreas of a male with pancreatic adenocarcinoma. Both the PL45 and the Panc 10.05 cell lines exhibit a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655 ] The cells have a reported plating efficiency of 40%. [50655 ] The Panc 10.05 cell line was derived from the same patient as the PL45 cell line (ATCC CRL-2558 ). |
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| Propagation: | ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15% Temperature: 37.0°C |
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| Subculturing: | Protocol:
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 19.2 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 derived from same individual:ATCC CRL-2558 |
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| References: | 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602 | ||
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文献和实验0.05mol/L Tris-HCl缓冲液（pH7.19～9.10）
12.0 18.0 8.05 10.0 11.0
，这种时候，有两种情况，一是这个 idea 真的很好，但大家总会忽略 N 年前就有人做过，当然，更多时候，觉得 idea 好，不过是一种错觉而已。 03 下周之前，论文就能投稿了三月许下的宏愿，直到九月才把论文投出去，从以为可以投稿，到投稿，往往有半年以上的距离。 04 这个实验快了，15 分钟后可以去吃饭论鸽王是怎么炼成的，自己心里没点数吗？ 05 新来的小师妹，是不是喜欢我我觉得隔壁实验室的大师姐，也挺喜欢你。 06 师兄毕业前发了 5 篇论文，我也可以学过生物的都知道，世界上没有两片相同的叶子
科学界一直以来都有这样的传统：如果科学家找不到“小白鼠”，或者觉得让他人冒险很不道德，那么就会自己“献身”科学。自我实验就像一首英雄主义的史诗，不过也散发着一丝丝疯狂的气息。下面展示的这10个疯狂的自我实验，看来也是一个比一个癫狂。 10. 音速急刹车 第二次世界大战后，美国空军需要知道飞行员从超音速飞机中弹后能否逃过一劫，因为由音速到接近零的突然减速往往会导致死亡。这种速度的变化会让飞行员受到超过40G甚至50G的压力。（1G
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