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- 细胞形态：
淋巴样
- 器官来源：
外周血
- 运输方式：
冻存运输
- ATCC Number：
CRL-2369™
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
B淋巴细胞
- 生长状态：
悬浮生长
| Designations: | C1R-neo | ||
| Depositors: | F Grumet | ||
| Biosafety Level: | 2 [Cells contain herpesvirus ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
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| Source: | Organ: peripheral blood Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 6,10 D13S317: 11,13 D16S539: 9,13 D5S818: 10,13 D7S820: 7,12 THO1: 8 TPOX: 8 vWA: 17 |
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| Comments: | C1R-neo is a stable transfectant cell line established in 1987 by transfection by electroporation of the C1R cell line with a modified neomycin drug-resistant eukaryotic vector, pSP65-Neo. The vector did not carry an insert. [38581 ] C1R-neo does not secrete or express HLA B7. C1R is a human B-cell lymphoblastoid line lacking surface HLA A and B antigens. C1R was derived from Hmy.2 B-LCL by gamma irradiation followed by selection for Class I monoclonal antibodies and complement. [23312 ] C1R-neo may be used as a control for C1R-sB7 (CRL-2370), in CTL experiments and for producing control supernatants. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 X 10(5) viable cells/ml. Maintain cultures at cell concentrations between 5 X 10(5) and 2 X 10(6) viable cells/ml. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005 recommended serum:ATCC 30-2020 parental cell line:ATCC CRL-1993 derived from same cell line:ATCC CRL-2370 derived from same cell line:ATCC CRL-2371 |
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| References: | 23312: Storkus WJ, et al. Reversal of natural killing susceptibility in target cells expressing transfected class I HLA genes. Proc. Natl. Acad. Sci. USA 86: 2361-2364, 1989. PubMed: 2784569 38581: Grumet FC, et al. Soluble form of an HLA-B7 class I antigen specifically suppresses humoral alloimmunization. Hum. Immunol. 40: 228-234, 1994. PubMed: 7960967 38582: Hiraki DD, et al. Bioengineered soluble HLA-B7. Genesis, characterization, and occurrence of dimerization. Hum. Immunol. 40: 235-246, 1994. PubMed: 7960968 |
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文献和实验新拉马克主义 neo－ Lamarckism 由帕卡德（ A． S． Packard）创造的，作为发展拉马克学说所提出的进化学说的总称。在十九世纪进化论确立之后，法国、美国形成了这一强有力的学派。这个学派以获得性遗传的主张为中心，但是拉马克学说本身包含有多种要素，所以新拉马克学派的学者中间也有各种见解。例如科普（ E． D． Cope）认为生物体具有其固有的作为进化原动力的生长力（ gr－ owth force）。 N geli（ C． N geli）也以相似的观点提出“完成化原理
新达尔文主义 neo-Darwinism 魏斯曼（ A． Weismann）仅强调达尔文学说中的生存斗争原理（参见自然选择万能），对有关达尔文的变异及其遗传的理论进行了修改，也就是在他的种质论（种质为独立的和连续的思想）的基础上，对获得性遗传，也就是说对拉马克学说的精髓进行了彻底的否定，这就是新达尔文主义。但现代的自然选择学说，从其观念的发展来看，也可以叫做新达尔文主义，而现在则主要是指此而言。
Characterization of a PGA-Based Scaffold for Use in a Tissue-Engineered Neo-Urinary Conduit
testing) and biological characterization (cell viability and proliferation) of a polyglycolic acid-based scaffold used to tissue engineer a Neo-Urinary Conduit™. Such methods are more broadly applicable to characterization of other neo-organ product
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