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- 生长状态：
贴壁生长
- 器官来源：
胰腺
- 运输方式：
冻存运输
- 细胞形态：
上皮样
- 相关疾病：
腺癌
- ATCC Number：
CRL-2554™
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 年限：
64 years
| Designations: | Panc 02.13 | ||
| Depositors: | EM Jaffee | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens deposited as human | ||
| Morphology: | epithelial |
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| Source: | Organ: pancreas Disease: adenocarcinoma |
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| Cellular Products: | cytokeratins 7 and 18 [50655 ] | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Oncogene: | K-ras + (wild-type) | ||
| Antigen Expression: | MHC class I +; MHC class II - [50655 ] Blood type A; Rh+ |
||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 8,11 D16S539: 9,12 D5S818: 12 D7S820: 7,11 THO1: 6,9.3 TPOX: 8 vWA: 16,18 |
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| Age: | 64 years | ||
| Gender: | female | ||
| Ethnicity: | White | ||
| Comments: | Panc 02.13 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma. The cells have a reported plating efficiency of 100%. [50655 ] |
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| Propagation: | ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15% Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Doubling Time: | 33.9 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602 | ||
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文献和实验人基质金属蛋白酶 -13 （ MMP-13 ） 酶联免疫分析 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 基质金属蛋白酶-13 （MMP-13 ） 的含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中人基质金属蛋白酶 13 （ MMP-13 ) 水平。用纯化的人基质金属蛋白酶 13 （ MMP-13 ) 抗体包被微孔板，制成固相抗体，往包
人基质金属蛋白酶 -13 （ MMP-13 ） 酶联免疫分析 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 基质金属蛋白酶-13 （MMP-13 ） 的含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中人基质金属蛋白酶 13 （ MMP-13 ) 水平。用纯化的人基质金属蛋白酶 13 （ MMP-13 ) 抗体包被微孔板，制成固相抗体，往包
接受审稿人的意见，进行一对一的修改。 12. Revised Manuscript Submitted： 意味着作者已经递交修改稿，现等待期刊检查排版。 13. Accept： 意味着编辑对作者的修改很满意，决定接受稿件。 二、审稿的一般流程 一篇 SCI 学术论文稿件在提交后，首先经过编辑对稿件是否符合所投期刊的定位以及稿件的创新性等进行初步评价。 如果不符合期刊定位或整体评价不高，编辑则会不经过同行评审，直接拒掉。 如果编辑初审认为稿件值得进一步评价，则就会进入同行评审阶段。编辑会随机或根据稿件中
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