- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 是否是肿瘤细胞:
0
- 运输方式:
冻存运输
- ATCC Number:
CRL-10860™
| Designations: | BTI-TN-MG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Morphology: | |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| This material is cited in a U.S. and/or other Patent or Patent Application, and may not be used to infringe on the patent claims. ATCC is required to inform the Patent Depositor of the party to which the material was furnished. |
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| Propagation: | ATCC complete growth medium: | ||
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文献和实验干货 | CUT and Tag 核心酶 Hyperactive PA/PG-Tn5 Transposase 大解密
PA/PG 寻找不同来源的抗体,结合在靶蛋白的附近后再进行切割。它的切割作用由 Tn5 转座酶实现,Tn5 转座酶作用机制主要分为三步: 01 转座酶(Tnp)分子(红色)与转座元件末端与特定的 19 bp ES 识别序列(白条)结合,形成两个 TnP-ES 复合体。随后两个 TnP-ES 复合体通过 TnP 的 C 末相互作用进行联会,形成 Tn5 转座酶复合体。 02 在 Mg2+ 存在下,Tnp 活化水分子,活化的水分子进行亲核攻击,水解 DNA
Single Primer ("Semi-Random") P
method is that the product has unique ends, allowing convergent sequencing. When amplifying out of inverted repeats at the ends of certain elements such as Tn10 or Tn5, gel purify bands before sequencing. If you try to sequence the crude PCR reaction
。 9. CUT&RUN 与 CUT&Tag 实验的主要区别是什么? CUT&RUN 中的功能酶是融合酶 pA-MNase ,使用 Ca2+ 激活,在 0℃ 切割。CUT&RUN 实验中的二抗为选择项。回收到的 DNA 为片段化的 DNA ,后续需要接传统的 DNA 文库构建,才能得到 CUT&RUN 文库。CUT&Tag 中的功能酶是融合酶 pA/pG-Tn5 ,该酶由 Mg2+ 在 37℃ 激活转座酶切割 DNA ,实验时需要加入二抗进行信号放大。回收到的 DNA 已经带有测序引物结合
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