H25B10产品图

H25B10

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  • 2026年06月28日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 物种来源

      小鼠

    • 是否是肿瘤细胞

      0

    • 库存

      大量

    • 免疫类型

      IgG1

    • 细胞形态

      淋巴样

    • ATCC Number

      CRL-8017™

    • 生长状态

      悬浮生长

    • 运输方式

      冻存运输

    Designations: H25B10
    Depositors:  Massachusetts General Hospital
    Isotype: IgG1
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: suspension
    Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
    Morphology: lymphoblast

    Source: Cell Type: hybridoma: B lymphocyte;
    Cellular Products: immunoglobulin; monoclonal antibody; against hepatitis B virus surface antigen (HBsAg)
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Comments: Animals were immunized with hepatitis B virus surface antigen.
    Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
    Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 85%; fetal bovine serum, 15%
    Subculturing: Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    References: 3495: Wands JR, Zurawski VR Jr.. Process for producing antibodies to hepatitis virus and cell lines therefor. US Patent 4,271,145 dated Jun 2 1981
    28236: Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997. PubMed: 8985336

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    图标文献和实验
    相关实验
    • Identifying Regulatory B Cells (B10 Cells) that Produce IL-10 in Mice

      -type mice. We call these cells B10 cells because they are responsible for most B cell IL-10 production, they appear to only produce IL-10 after 5 h of in vitro stimulation, and to distinguish them from other potential regulatory B cell subsets. B10

    • Murine Natural Killer Cells and Hybrid Resistance to Hemopoietic Cells In Vivo

      a “good responders” “poor responders” H2 b/b B10.D2, NZB, B10.D2 x DBA/2 BALB/c, DBA

    • 菌绿素 bacteriochlorophyll

      -650),与少量菌绿素 a同时存在于绿色的光合细菌中,它们都可各自分成多种结构极近似的成分。菌绿素 c和 d,与茵绿素 a和 b类似,可以看成是二氢卟吩的衍生物,但是前者不能和叶绿醇形成酯、而是和法泥醇( C15 H25 OH)形成酯。菌绿素的光吸收带,在活细胞中较之在有机溶剂中,更偏向长波长区。例如茵绿素 a在乙醚中主要吸收带在 357.5和 770纳米波长处,而在活细胞中,变成在 380, 890, 870, 850, 800纳米附近表现极大的光吸收。各种起同化作用色素吸收的光能只有通过菌

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