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- 2025年10月08日
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- 详细信息
- 文献和实验
- 技术资料
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 库存：
大量
- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 相关疾病：
肿瘤
- ATCC Number：
CRL-2578™
- 器官来源：
结肠
| Designations: | RKO-E6 | ||
| Depositors: | MC Hollander, AJ Fornace | ||
| Biosafety Level: | 2 [Contains HPV6 and CMV viral sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens deposited as human | ||
| Morphology: | epithelial |
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| Source: | Organ: colon Disease: carcinoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | The RKO-E6 cell line was generated from the colon carcinoma RKO (ATCC CRL-2577 ) cell line by transfection with pCMV-E6 using Lipofectin. [53390 ] The cells contain a stably integrated human papilloma virus (HPV) E6 oncogene under control of the cytomegalovirus (CMV) promoter. The HPV E6 oncogene causes a decrease in normal p53 levels and functions. The RKO-E6 cell line lacks appreciable functional p53. Disruption of normal p53 function in human colon carcinoma RKO cells with the human papillomavirus E6 oncoprotein results in reduced repair of u.v.-induced DNA damage and also loss of induced repair following cellular u.v.-irradiation. [51463 ] RKO cells contain wild-type p53 but lack endogenous human thyroid receptor nuclear receptor (h-TRbeta1). The level of p53 protein is higher in RKO (ATCC CRL-2577 ) cells than in RKO-E6 (ATCC CRL-2578) cells. [53331 ] The RKO-E6 cell line can be used together with its parental cell line, RKO (ATCC CRL-2577 ) to investigate the effects of p53 loss on cellular parameters such as p53 mediated transcription and apoptosis. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 parental cell line:ATCC CRL-2577 |
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| References: | 51463: Smith ML, et al. Involvement of the p53 tumor suppressor in repair of u.v.-type DNA damage. Oncogene 10: 1053-1059, 1995. PubMed: 7700629 51464: Smith ML, et al. Antisense GADD45 expression results in decreased DNA repair and sensitizes cells to u.v.-irradiation or cisplatin. Oncogene 13: 2255-2263, 1996. PubMed: 8950993 53331: Bhat MK, et al. Tumor suppressor p53 is a negative regulator in thyroid hormone receptor signaling pathways. J. Biol. Chem. 272: 28989-28993, 1997. PubMed: 9360971 53390: Kessis TD, et al. Human papillomavirus 16 E6 expression disrupts the p53-mediated cellular response to DNA damage. Proc. Natl. Acad. Sci. USA 90: 3988-3992, 1993. PubMed: 8387205 |
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文献和实验红的是E6的开放读码，兰色的是E7的开放读码，中间有几个碱基，在基因组中E6和E7就是这样排列的， 请教各位高手，我如果简单的把上述E6E7的完整片段插到真核表达系统，能否做到E6E7的共同表达？如何分析，请指教 关于双基因的表达问题：我查了一些资料，里面很有学问。
In Vitro Assays of Substrate Degradation Induced by High-Risk HPV E6 Oncoproteins
The high-risk mucosal human papillomavirus E6 proteins were the first viral proteins that were shown to use the ubiquitin proteasome pathway for the inactivation of their cellular target proteins. The first substrate to be identified
Immortalization of Human Prostate Cells With the Human Papillomavirus Type 16 E6 Gene
A survey of the Medline titles for the years 2000–2001 revealed 125 papers whose titles included the name of the androgen-sensitive prostate cancer cell line LNCaP, and 36 papers also included PC3, the most common androgen independent line
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