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- 库存：
大量
- 运输方式：
冻存运输
- 物种来源：
褐鼠
- 是否是肿瘤细胞：
0
- ATCC Number：
HB-9478™
- 免疫类型：
IgG2a
- 细胞形态：
淋巴样
- 生长状态：
悬浮生长
| Designations: | MECA-367 | ||
| Depositors: | Stanford University Board of Trustees | ||
| Isotype: | IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Rattus norvegicus (B cell); Mus musculus (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against mouse Peyer's patch endothelial cells (MAdCAM-1) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with mouse lymphnode stroma. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody specifically blocks lymphocyte adhesion to mouse Peyer's patch endothelia and does not block lymphocyte binding to peripheral lymph nodes. It recognizes the N terminal domain of mouse mucosal vascular addressin (MAdCAM-1) involved in alpha 4, beta 7 (a4b7) integrin binding. |
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| Propagation: | ATCC complete growth medium: HB101 medium, 100% - OR - RPMI 1640 medium, 90%; fetal bovine serum, 10% Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| References: | 22068: Butcher EC. Method to control leukocyte extravasation. US Patent 5,403,919 dated Apr 4 1995 22376: Streeter PR, et al. A tissue-specific endothelial cell molecule involved in lymphocyte homing. Nature 331: 41-46, 1988. PubMed: 3340147 22636: Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523 22937: Briskin MJ, et al. MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1. Nature 363: 461-464, 1993. PubMed: 8502297 |
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文献和实验本文由丁香园站友 sunxjk 于2011-3-3 转载， 点此 参与论坛讨论 3月2日，清华大学生命科学院施一公教授领导的研究组与王佳伟副教授合作在《自然》在线发表论文，报道原核细胞蛋白酶体调节亚基MecA-ClpC异六聚体结构与功能的研究。 ATP依赖的可调控蛋白质水解广泛存在于大多数生命体中，对于及时清除机体内的垃圾蛋白以及调节蛋白具有十分重要的作用。原核
by the mecA gene in S. aureus . Because routine laboratory diagnostics may be time consuming and because species differentiation encounters a variety of difficulties, molecular techniques detecting both the mecA and a S. aureus -specific gene are used
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