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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 运输方式:
冻存运输
- 生长状态:
悬浮生长
- 物种来源:
小鼠
- 是否是肿瘤细胞:
0
- 年限:
8 months
- 细胞形态:
淋巴样
- 相关疾病:
其他疾病
- ATCC Number:
CCL-219™
- 品系:
DBA subline 212
- 库存:
大量
| Designations: | L1210 | ||
| Depositors: | EM Jensen | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus | ||
| Morphology: | lymphoblast |
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| Source: | Disease: lymphocytic leukemia Strain: DBA subline 212 |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Cytogenetic Analysis: | modal number = 40; range = 32 to 80. Mouse karyotype with a diploid stemline number of 40 chromosomes. |
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| Age: | 8 months | ||
| Gender: | female | ||
| Comments: | Tested and found negative for ectromelia virus (mousepox). The line was established from a tumor in female #234 that developed following skin paintings with 0.2% methylcholanthrene in ether. [22513 ] |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 viable cells/ml. Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 4 days |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 8 to 10 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2040 |
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| References: | 22513: . . J. Natl. Cancer Inst. 10: 179-192, 1949. 26055: . . J. Natl. Cancer Inst. 36: 405-421, 1966. 26056: . . Cancer Chemother. Rep. 51: 451-453, 1967. 32923: Young SW, et al. Gadolinium(III) texaphyrin: a tumor selective radiation sensitizer that is detectable by MRI. Proc. Natl. Acad. Sci. USA 93: 6610-6615, 1996. PubMed: 8692865 |
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文献和实验一光年 最近要做生殖的细胞方面的实验,前期一直用在垂体细胞原代培养,但是一直困扰没有促性腺激素细胞系,但是在网络中搜索,发现老外的文章中经常提到αT3-1,LβT2 或LβT4细胞系,在ATCC等知名的细胞库也未发现这些细胞系,不知道各位高手指点在哪里可以找到这几种细胞系?不甚感激!! veimojie 我一直也在做这方面的研究 什么地方能有啊 ? banana_smile 我也想做这方
仟岛除湿机BD-1210 仟岛除湿机BD-1210 型号:BD-1210 额定除湿能力:10升/日 适用环境温度:5-35℃ 功率:250W 净重:8.45kg 外形尺寸:308×213×534mm 适用面积:10-30m2 水箱容量:2升 ☆ 超静高效 ☆ 大抽湿量节能 ☆ 后置水箱,水箱经纳米灭菌处理 ☆ 配可移动转轮 ☆ 连续排水孔,可接排水管 ☆ 前置出风口 ☆ 水满防溢自动停机 ☆ 断电
发现于肝脏和酵母中,是鼠乳汁分泌的必需因子( L因子,泌乳因子)(中原和郎, 1934),对人的效果不肯定,以后清楚此因子无论其化学结构还是作用机理都是由二种不同的维生素 L1 邻氨基苯甲酸( anth- ranilic acid)和 L2 (腺嘌呤硫代甲基戊糖)组成的(中原和郎, 1945)。但 L效果没有确实的证明,因此把它作为独立的维生素看来是有问题的。
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