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- ATCC Number：
HB-9119™
- 运输方式：
冻存运输
- 免疫类型：
IgG3
- 细胞形态：
淋巴样
- 库存：
大量
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 生长状态：
悬浮生长
| Designations: | P25.48 | ||
| Depositors: | Sloan-Kettering Institute for Cancer Research | ||
| Isotype: | IgG3 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human prostate cancer | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with fresh prostate cancer (tumor P251). Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with malignant prostatic epithelium but not with benign prostate epithelium. |
||
| Propagation: | ATCC complete growth medium: RPMI 1640 medium with non-essential amino acids, 2 mM L-glutamine, 0.2 mM hypoxanthine and 0.032 mM thymidine, 90%; fetal bovine serum, 10% |
||
| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| References: | 21955: Bazinet M, et al. Monoclonal antibodies selective for prostate cancer. US Patent 4,970,299 dated Nov 13 1990 | ||
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文献和实验CD48 分子 CD48 常用单克隆抗体或代号： WM68,LO- MN25;(BLAST- 1,OX45,BCM- 1) 主要表达细胞： Leu [NL] 分子质量(kDa)和结构： gp45,(GPI连接)(IgSF) 功 能： CD2的配体(小鼠,大鼠),人CD48与CD2结合力低 CD48 Mediates adhesion via its receptor, CD
Preparing 48-Well Plate Feeders
Preparing 48-Well Plate Feeders In preparing 48-well plates, a dilution must be made from the regular density of 3.5 x 105 cells/ml. The required density for 48-well plates is: 8.2 x 104 cells/ml (650 ml/well or 31.2 mls
凉凉！最高 12 分，今年多达 48 本 SCI 被镇压......
北京时间 6 月 29 日，科睿唯安发布了 2020 年度《期刊引证报告》（JCR），涨涨跌跌的影响因子，可谓是牵动着广大科研狗的心。图片来源：科睿唯安影响因子一经发布，自然是几家欢喜几家愁，有人论文刚接收，就迎来影响因子上涨的好消息，可谓是双喜临门；也有人恰恰相反，文章还没 online，期刊影响因子就掉了不少；但这还不是最惨的，最惨的事情莫过于，论文接收时，期刊还是 SCI，影响因子发布后，期刊就被无情镇压。图片来源：科睿唯安这样的事情，每年都在发生，2020 年，自然也不例外。图片来源
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