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- 相关疾病：
淋巴瘤
- ATCC Number：
CRL-1588™
- 细胞形态：
淋巴样
- 库存：
大量
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 品系：
AKR/J
- 细胞类型：
T淋巴细胞
- 生长状态：
悬浮生长
- 运输方式：
冻存运输
| Designations: | BW5147.G.1.4.OUAR.1 | ||
| Depositors: | R Hyman | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus | ||
| Morphology: | lymphoblast |
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| Source: | Disease: lymphoma Strain: AKR/J Cell Type: T lymphocyte; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Antigen Expression: | H-2k | ||
| Comments: | The cells are ouabain resistant. They can be used to create T cell hybridomas. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; horse serum, 10% |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 3 X 10 exp4 viable cells/ml. Subculture every 3 to 4 days. |
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| References: | 22529: Hyman R, Stallings V. Complementation patterns of Thy-1 variants and evidence that antigen loss variants "pre-exist" in the parental population. J. Natl. Cancer Inst. 52: 429-436, 1974. PubMed: 4544550 | ||
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文献和实验Generation of T Cell Hybridomas from Naturally Occurring FoxP3+ Regulatory T Cells
Generation of regulatory T cells (or Treg) derived hybridomas offers a tool to study their antigen specificity. T cells hybridomas are produced by fusing TCR α-β-thymoma BW5147 with highly dividing T cell population. In vitro anergy of Tregs
IM: 肌肉注射 IV: 静脉注射 SC: 皮下注射 IP: 腹腔注射药 物剂量途径麻醉前给药Atropine0.002-0.005 mg/100 g BW0.12 mg/100 g BWIM, IV, SC*IP*镇静剂AcepromazineDiazepamKetamine0.075 mg/100 g BW0.5 mg/100 g BW2.0 mg/100 g BWIMIPIM注射
0.002-0.005 mg/100 g BW 0.12 mg/100 g BW IM, IV, SC * IP * 镇静剂 Acepromazine Diazepam
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